Carbonic anhydrase-IN-35 

Carbonic anhydrase-IN-35 is a selective carbonic anhydrase (CA) inhibitor. Carbonic anhydrase-IN-35 potently inhibits tumor-associated hCA IX (K_i = 0.6 nM) and hCA XII (K_i = 2.2 nM). Carbonic anhydrase-IN-35 induces apoptosis in MCF-7 cells by elevating Bax, reducing Bcl-2, and downregulating CDK4/6. Carbonic anhydrase-IN-35 exhibits potent cytotoxicity against MCF-7 (IC₅₀ = 0.3975 μM normoxic/0.6575 μM hypoxic), MCF-7-ADR (IC_{50> = 0.3975 μM normoxic/4.488 μM hypoxic), MDA-MB-231, and 4T1 breast cancer cells. Carbonic anhydrase-IN-35 can be used for the study of breast cancer^[1].}

Carbonic anhydrase-IN-35 (Compound 10a) 对肿瘤相关 hCA IX (K_i = 0.6 nM)、hCA XII (K_i = 2.2 nM)、hCA I (K_i = 6183 nM) 和 hCA II (K_i = 12.7 nM) 表现出强效抑制活性^[1]。
Carbonic anhydrase-IN-35 在常氧条件下对 MCF-7 细胞显示强效细胞毒性 (IC₅₀ = 0.3975 μM) 和低氧条件下 (IC₅₀ = 0.6575 μM)^[1]。
Carbonic anhydrase-IN-35 在常氧条件下对 MCF-7-ADR 乳腺癌细胞显示强效细胞毒性 (IC₅₀ = 0.3975 μM) 和低氧条件下 (IC₅₀ = 4.488 μM)^[1]。
Carbonic anhydrase-IN-35 对 MDA-MB-231 三阴性乳腺癌细胞显示强效抗增殖活性 (IC₅₀ = 1.9 μM) 和对 4T1 三阴性乳腺癌细胞 (IC₅₀ = 2.38 μM)^[1]。
Carbonic anhydrase-IN-35 (24 h) 在 MCF-7 乳腺癌细胞中增加促凋亡因子 Bax 的表达，下调抗凋亡因子 Bcl-2 和 CDK4/6^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

400.41

C₁₇H₁₆N₆O₄S

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ibrahim MH, et al. Sulfonamide-arylidene hybrids featuring flexible hydrazide and rigid triazole linkers: design, synthesis, and evaluation as carbonic anhydrase inhibitors with anti-breast cancer potential. Bioorg Chem. 2025 Jul 29;164:108803.  [Content Brief]