2. PI3K/Akt/mTOR
  3. mTOR
  4. eCF309

eCF309 是一种强效且高选择性的 mTOR 抑制剂，IC50 为 15 nM。eCF309 比 PI3K 具有更高的选择性。eCF309 可用于乳腺癌和前列腺癌的研究。

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eCF309

eCF309 Chemical Structure

CAS No. : 2001571-40-8

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规格 价格 是否有货 数量
500 μg ￥1100
In-stock
1 mg ￥1700
In-stock
5 mg   询价  
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Customer Review

eCF309 相关抗体

Top Publications Citing Use of Products

查看 mTOR 亚型特异性产品:

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mTOR mTORC1 mTORC2

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

eCF309 is a potent and highly selective mTOR inhibitor with an IC50 of 15 nM. eCF309 displays higher selectivity over PI3Ks. eCF309 can be used for the study of breast cancer and prostate cancer[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
MCF7 EC50
8 nM
Compound: 12
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
10.1039/C5MD00493D
MDA-MB-231 EC50
72 nM
Compound: 12
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
10.1039/C5MD00493D
PC-3 EC50
37 nM
Compound: 12
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
10.1039/C5MD00493D
T98 EC50
100 nM
Compound: eCF309
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
[PMID: 31787462]
U-87MG ATCC EC50
100 nM
Compound: eCF309
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
[PMID: 31787462]
体外研究
(In Vitro)

eCF309 (0.3-1000 nM; 5 days) exhibits highly potent antiproliferative activity against MCF7, MDA-MB-231 and PC3 cells[1].
eCF309 (0.3-1000 nM; 24 h) causes G0/G1 cell cycle arrest[1].
eCF309 (3-100 nM; 1.5 h) inhibits pP70S6K, pS6 and pAKT levels in MCF7 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

eCF309 相关抗体:

Cell Viability Assay[1]

Cell Line: MCF7, MDA-MB-231 and PC3 cells
Concentration: 0.3-1000 nM
Incubation Time: 5 days
Result: Exhibits EC50 values upon MCF7, MDA-MB-231 and PC3 cells viability of 8.4 nM, 72 nM and 37 nM, respectively.

Cell Cycle Analysis[1]

Cell Line: MCF7, MDA-MB-231 and PC3 cells
Concentration: 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM, 300 nM, 1000 nM
Incubation Time: 24 h
Result: Caused G0/G1 cell cycle arrest.

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 3 nM, 10 nM, 30 nM, 100 nM
Incubation Time: For 30 min, followed by 1 h of serum stimulation
Result: Mediated a dose-dependent reduction of the pP70S6K, pS6 and pAKT levels in MCF7 cells, achieving almost complete inhibition of the phosphorylation of these targets at 30 nM.
分子量

383.40

Formula

C18H21N7O3
CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 1 mg/mL (2.61 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6082 mL 13.0412 mL 26.0824 mL
5 mM --- --- ---
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.2%

COA (195 KB)

产品使用指南 (1538 KB)
参考文献

eCF309 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6082 mL 13.0412 mL 26.0824 mL 65.2061 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

eCF3092001571-40-8eCF 309eCF-309mTORMammalian target of RapamycinMCF-7HER2breast cancerInhibitorinhibitorinhibit

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目录号：
HY-128027
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