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  3. eGFP mRNA(N1-Me-Pseudo UTP)

eGFP mRNA(N1-Me-Pseudo UTP) 

目录号: HY-153079 纯度: 97.00%
COA 产品使用指南 技术支持

eGFP mRNA 可以表达绿色荧光蛋白,最初从水母 Aequorea victoria中分离出来。eGFP 是哺乳动物细胞培养中常用的直接检测报告蛋白,在509 nm处产生明亮的绿色荧光。

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eGFP mRNA(N1-Me-Pseudo UTP) Chemical Structure

eGFP mRNA(N1-Me-Pseudo UTP) Chemical Structure

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规格 价格 是否有货 数量
100 μg (1 mg/mL * 100 μL in Water) ¥3200
In-stock
500 μg (1 mg/mL * 500 μL in Water) 现货 询价
1 mg (1 mg/mL * 1 mL in Water) 现货 询价

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

The eGFP mRNA will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

体外研究
(In Vitro)

用未修饰、me1 ψ-、5 moU- 或 ψ-修饰的 eGFP mRNA 转染 Hep 3B 细胞。处理 1 小时后,以不同时间间隔 (t = 0、1.5、3 和 4.5 小时) 收集总 RNA,并逆转录成互补 DNA (cDNA)。通过定量 PCR (qPCR) 分析,以内源性 18S rRNA 为标准化标准,计算每个 eGFP mRNA 的含量[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.

储存方式

-80°C

纯度 & 产品资料

纯度: 97.00%

参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
eGFP mRNA(N1-Me-Pseudo UTP)
目录号:
HY-153079
需求量: