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  4. Enhanced Green Fluorescent Protein (EGFP) (200-208)

Enhanced Green Fluorescent Protein (EGFP) (200-208) 

目录号: HY-P2528 纯度: 98.97%
COA 产品使用指南 技术支持

Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种标记基因产物，源自维多利亚水母 (Aequorea Victoria)。Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种常见的报告蛋白，易于被检测到。

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Custom Peptide Synthesis

Enhanced Green Fluorescent Protein (EGFP) (200-208)

Enhanced Green Fluorescent Protein (EGFP) (200-208) Chemical Structure

CAS No. : 323198-39-6

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥550
In-stock
5 mg ￥1500
In-stock
10 mg ￥2400
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Enhanced Green Fluorescent Protein (EGFP) (200-208) 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect[1].

体外研究
(In Vitro)

Enhanced Green Fluorescent Protein (EGFP) (200-208) 是增强型绿色荧光蛋白 (EGFP) 的一个特异性肽段，含有 9 个氨基酸，是 EGFP 的免疫显性表位。Enhanced Green Fluorescent Protein (EGFP) (200-208) 可由 H-2Kd 分子呈递。EGFP 200-208-H-2Kd 复合物可作为 T 细胞的抗原，被 T 细胞受体 (TCR) 特异性识别并杀伤[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Enhanced Green Fluorescent Protein (EGFP) (200-208) 相关抗体:
分子量

1019.11

Formula

C45H70N12O15
CAS 号
性状

固体

颜色

White to off-white

Sequence

His-Tyr-Leu-Ser-Thr-Gln-Ser-Ala-Leu
Sequence Shortening

HYLSTQSAL
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
细胞实验： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献

Enhanced Green Fluorescent Protein (EGFP) (200-208) 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9812 mL 4.9062 mL 9.8125 mL 24.5312 mL
5 mM 0.1962 mL 0.9812 mL 1.9625 mL 4.9062 mL
10 mM 0.0981 mL 0.4906 mL 0.9812 mL 2.4531 mL
15 mM 0.0654 mL 0.3271 mL 0.6542 mL 1.6354 mL
20 mM 0.0491 mL 0.2453 mL 0.4906 mL 1.2266 mL
25 mM 0.0392 mL 0.1962 mL 0.3925 mL 0.9812 mL
30 mM 0.0327 mL 0.1635 mL 0.3271 mL 0.8177 mL
40 mM 0.0245 mL 0.1227 mL 0.2453 mL 0.6133 mL

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Enhanced Green Fluorescent Protein (EGFP) (200-208)323198-39-6Fluorescent DyeInhibitorinhibitorinhibit

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产品名称：
Enhanced Green Fluorescent Protein (EGFP) (200-208)
目录号：
HY-P2528
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