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  2. Enhanced Green Fluorescent Protein (EGFP) (200-208)

Enhanced Green Fluorescent Protein (EGFP) (200-208) 

目录号: HY-P2528
产品使用指南

Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种标记基因产物,源自维多利亚水母 (Aequorea Victoria)。Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种常见的报告蛋白,易于被检测到。

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Custom Peptide Synthesis

Enhanced Green Fluorescent Protein (EGFP) (200-208) Chemical Structure

Enhanced Green Fluorescent Protein (EGFP) (200-208) Chemical Structure

CAS No. : 323198-39-6

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Description

Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect[1].

Molecular Weight

1019.11

Formula

C₄₅H₇₀N₁₂O₁₅

CAS No.

323198-39-6

Sequence Shortening

HYLSTQSAL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Enhanced Green Fluorescent Protein (EGFP) (200-208)OthersInhibitorinhibitorinhibit

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产品名称:
Enhanced Green Fluorescent Protein (EGFP) (200-208)
目录号:
HY-P2528
需求量: