Glycylglycine hydrochloride hydrate  (Synonyms: Diglycine hydrochloride hydrate; Gly-Gly (HCl H2O))

Glycylglycine hydrochloride hydrate is a non-selective Glycylglycine hydrochloride hydrate dipeptidase substrate and iNOS inhibitor. Glycylglycine hydrochloride hydrate can cross the cell membrane by passive diffusion and is hydrolyzed to glycine in the cell, participating in energy metabolism and antioxidant processes. Glycylglycine hydrochloride hydrate promotes spermatogonial stem cells (SSCs) proliferation, inhibits astrocyte overactivation and reduces nitric oxide (NO) release, while upregulating the expression of neurotrophic factors (such as PDGFA, FGF2, CNTF) to support nerve myelin repair. Glycylglycine hydrochloride hydrate can be used to study male reproductive biology (such as SSCs proliferation regulation) and neurodegenerative diseases (such as neuroprotective mechanisms in multiple sclerosis)^[1][2].

细胞活力实验：
Glycylglycine hydrochloride hydrate (10 μM；5 d) 在精原干细胞 (SSCs) 培养中显著促进细胞增殖，细胞活力提高约 50%，且对细胞凋亡和细胞周期无显著影响^[1]。
Glycylglycine hydrochloride hydrate (0.1-1 mM；24 h) 抑制星形胶质 U87 细胞生长，且 Glycylglycine hydrochloride hydrate (1 mM；12-72 h) 对 DNA 合成无显著影响^[2]。
炎症因子检测实验：
Glycylglycine hydrochloride hydrate (0.5 mM；24 h) 在星形胶质 U87 细胞中显著抑制一氧化氮 (NO) 释放，降低炎症反应，同时上调神经营养因子 (如 PDGFA、FGF2、CNTF) 的表达，促进神经保护和修复^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

186.59

C₄H₁₁ClN₂O₄

23273-91-8

甘氨酸盐酸盐一水合物； Gly-Gly盐酸盐一水合物

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Xu B, et al. Glycylglycine plays critical roles in the proliferation of spermatogonial stem cells. Mol Med Rep. 2019 Oct;20(4):3802-3810.  [Content Brief]

  [2]. Zhao C, et al. Metabolomic analysis revealed glycylglycine accumulation in astrocytes after methionine enkephalin administration exhibiting neuron protective effects. J Pharm Biomed Anal. 2015 Nov 10;115:48-54.  [Content Brief]