1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Apoptosis and Necrosis
  5. Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit

Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit 

Annexin V-mCherry/SYTOX Green 细胞凋亡检测试剂盒

Cat. No.: HY-K1077
Manual SDS

Annexin V-mCherry/SYTOX Green 细胞凋亡检测试剂盒可方便、快捷的检测细胞凋亡。经 Annexin V-mCherry 和 SYTOX Green 联合染色后,正常细胞基本无荧光,凋亡细胞呈红色荧光,坏死细胞呈红色和绿色荧光。

Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 T ¥1235 In-stock
50 T ¥2467 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS is translocated from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with mCherry can identify apoptotic cells by binding to PS exposed on the outer leaflet. SYTOX Green is an excellent green-fluorescent nuclear and chromosome counterstain that only works on dead cells. After staining cells with Annexin V-mCherry and SYTOX Green, live cells show little or no fluorescence, apoptosis cells show red fluorescence, necrosis cells show red and green fluorescence.

Storage

-20°C, 1 year

Protect from light

Avoid repetitive freeze-thaw cycles

Protocol

1. Collect 1-5 × 105 cells

For suspension cells: Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

For adherent cells: Collect the cell culture medium. Wash cells with PBS and add trypsin to dissociate cells. Add the medium and gently suspend the cells to make a single-cell suspension. Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

Note: It is recommended to use trypsin containing no EDTA.

2. Resuspend the cells in 195 μL of Binding Buffer.

3. Add 5 μL of Annexin V-mCherry.

4. Add 1 μL of SYTOX Green.

5. Incubate the cells at room temperature for 10-20 minutes in the dark.

6. Detect the fluorescence by flow cytometer or fluorescence microscope. The maximum excitation wavelength and emission wavelength of mCherry were 587 nm and 610 nm respectively, the maximum excitation wavelength and emission wavelength of SYTOX Green-DNA were 504 nm and 523 nm respectively.

Components
Components HY-K1077-20T HY-K1077-50T
Annexin V-mCherry 100 μL 250 μL
Binding Buffer 12 mL 30 mL
SYTOX Green Stain 20 μL 50 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Annexin V-mCherry/SYTOX Green 细胞凋亡检测试剂盒
目录号:
HY-K1077
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