1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Apoptosis and Necrosis
  5. Apoptosis and Necrosis Assay Kit

Apoptosis and Necrosis Assay Kit 

细胞凋亡与坏死检测试剂盒

Cat. No.: HY-K1070
Manual SDS

MCE 细胞凋亡与坏死检测试剂盒采用 Hoechst 33342 和碘化丙啶(Propidium Iodide, PI)双染的方法快速检测细胞凋亡与细胞坏死。


Apoptosis and Necrosis Assay Kit

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
100T ¥390 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • components

  • Documentation

Description
& Advantages

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. This kit contains two ready-to-use dyes bound to DNA. Hoechst 33342, a type of blue fluorescence dye (excitation/emission maxima ≈ 352/461 nm when bound to DNA), stains the condensed chromatin in apoptotic cells more brightly than the chromatin in normal cells. Propidium Iodide (PI) is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids. The simultaneous use of these two dyes makes it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometer or fluorescence microscope. Normal cells show weak blue fluorescence + weak red fluorescence, apoptotic cells show strong blue fluorescence + weak red fluorescence, necrotic cells show strong blue fluorescence + strong red fluorescence.


This kit takes only 20-30 minutes to complete one-step staining and there is no need for dilution or preparation of other solutions when detected by flow cytometer. Each kit contains enough reagents for 100 samples.


Publications
Storage

4°C, 6 months; -20°C, 1 year


Protect from light


components
Components HY-K1070-100T
Cell Stain Buffer 100 mL
Hoechst 33342 Stain 0.5 mL
PI Stain 0.5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
细胞凋亡与坏死检测试剂盒
目录号:
HY-K1070
需求量: