2. MCE Kits
  3. Molecular Biology
  4. Vector Construction
  5. Seamless Cloning
  6. Seamless DNA Assembly Plus Kit

Seamless DNA Assembly Plus Kit 

无缝克隆试剂盒

Cat. No.: HY-K1041
Manual SDS

MCE Seamless DNA Assembly Plus Kit 无缝克隆试剂盒为新一代重组克隆试剂盒，一次反应可完成单个至多个 DNA 片段的重组。其中经优化的 Mix 预混了重组酶和反应缓冲液，并添加了辅助因子，可显著提高克隆的重组效率和对杂质的耐受度，最快仅需 5 分钟即可完成单片段重组，且阳性率高达 95% 以上。

Seamless DNA Assembly Plus Kit

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
50 rxns ￥1210 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5´ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.
Storage
Store at -20°C for 2 years. Avoid repeated freeze-thaw cycles.
Components
Components HY-K1041-50 rxns
Seamless Assembly Mix Plus 250 μL
pUC19 Control Plasmid, Linearized (Ampr, 40 ng/μL) 5 μL
500 bp Control Fragment (20 ng/μL) 5 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
无缝克隆试剂盒
目录号：
HY-K1041
需求量：
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