2. Apoptosis
  3. Apoptosis Caspase Bcl-2 Family
  4. Lobaplatin

Lobaplatin  (Synonyms: 络铂; D-19466)

目录号: HY-105930 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

Lobaplatin (D-19466) 是铂 (II) 络合物的非对映混合物。Lobaplatin 将细胞周期阻滞在 G1 和 G2/M 期。Lobaplatin 通过增加 caspase和 Bax 的表达，降低 Bcl-2 的表达来诱导细胞凋亡 (apoptosis)。Lobaplatin 可用于癌症研究。

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Lobaplatin Chemical Structure

Lobaplatin Chemical Structure

CAS No. : 135558-11-1

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥3740
In-stock
1 mg ￥1100
In-stock
5 mg ￥3363
In-stock
10 mg ￥4975
In-stock
25 mg ￥7960
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Lobaplatin 相关抗体

Top Publications Citing Use of Products

查看 Caspase 亚型特异性产品:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

查看 Bcl-2 Family 亚型特异性产品:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Lobaplatin (D-19466) is a diastereometric mixture of platinum(II) complexe. Lobaplatin arrests cell cycle at G1 and G2/M phase. Lobaplatin induces apoptosis by increasing expressions of caspase and Bax, decreasing expression of Bcl-2. Lobaplatin can be used for research of cancer[1][2][3].

体外研究
(In Vitro)

Lobaplatin (D-19466, 0.25-32 μg/mL; 24-72 h) exhibits anti-proliferative activities against esophageal squamous cell carcinoma (ESCC) cell lines[1].
Lobaplatin (0-16 μg/mL; 48 h) induces esophageal squamous cell carcinoma (ESCC) apoptosis and modulates expression of apoptosis-related proteins[1].
Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) arrests cell cycle progression at G1 and G2/M phases in a time-dependent manner[2].
Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) inhibits the mRNA levels of cyclin B, CDK1, and CDC25C phosphatase, down-regulates Rb/E2F complexes and up-regulates of CDK inhibitors[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Lobaplatin 相关抗体:

Cell Viability Assay[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0.25, 0.5, 1, 2, 4, 8, 16 and 32 μg/mL
Incubation Time: 24, 48 and 72 hours
Result: Inhibited the growth of KYSE-410 and EC-109 cells in a dose- and time-dependent manner.
Inhibited the clone formation activity of KYSE-410 and EC-109 cells in a dosedependent manner.

Apoptosis Analysis[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0.25, 1, 4 and 16 μg/mL
Incubation Time: 48 hours
Result: Increased the percentage of apoptotic cells in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0, 1, 4 and 16 μg/mL
Incubation Time: 48 hours
Result: Increased expressions of cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9 and Bax, while decreased expression of Bcl-2.

Cell Cycle Analysis[2]

Cell Line: SMMC-7721 cells
Concentration: 1.45 μg/mL
Incubation Time: 0, 24, 36 and 48 hours
Result: Arrested the proportions of G1, S, and G2/M phases in cells were 45.31, 22.88, and 31.81% at 0 h, 59.91, 11.92, and 28.17% at 24 h, 56.89, 2.83, and 40.28% at 36 h, and 53.80, 2.07, and 44.13% at 48 h, respectively.

Western Blot Analysis[2]

Cell Line: SMMC-7721 cells
Concentration: 1.45 μg/mL
Incubation Time: 0, 24, 36 and 48 hours
Result: Down-regulated cyclin B, CDK1, CDC25C, phosphorylated CDK1 (pCDK1), pCDK4, Rb, E2F, and pRb, and up-regulated p53, p21, and p27.
体内研究
(In Vivo)

Lobaplatin (5 and 10 mg/kg; i.p.; once a week, for 3 weeks) suppresses tumor growth of esophageal squamous cell carcinoma (ESCC) xenograft[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c nude mice (4-6 weeks) with ESCC xenograft[1]
Dosage: Intraperitoneal injection; once a week, for 3 weeks
Administration: 5 and 10 mg/kg
Result: Suppressed tumor volumes in a dose-dependent manner.
Increased expressions of Bax and decreased expressions of Bcl-2.
Clinical Trial
分子量

397.33

Formula

C9H18N2O3Pt
CAS 号
性状

固体

颜色

White to off-white

中文名称

络铂
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

H2O 中的溶解度 : 50 mg/mL (125.84 mM; 超声助溶 (<60°C); DMSO can inactivate Lobaplatin's activity)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5168 mL 12.5840 mL 25.1680 mL
5 mM 0.5034 mL 2.5168 mL 5.0336 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 100 mg/mL (251.68 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥98.0%

COA (270 KB) HNMR (256 KB) MS (68 KB)

产品使用指南 (1538 KB)
参考文献

Lobaplatin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.5168 mL 12.5840 mL 25.1680 mL 62.9200 mL
5 mM 0.5034 mL 2.5168 mL 5.0336 mL 12.5840 mL
10 mM 0.2517 mL 1.2584 mL 2.5168 mL 6.2920 mL
15 mM 0.1678 mL 0.8389 mL 1.6779 mL 4.1947 mL
20 mM 0.1258 mL 0.6292 mL 1.2584 mL 3.1460 mL
25 mM 0.1007 mL 0.5034 mL 1.0067 mL 2.5168 mL
30 mM 0.0839 mL 0.4195 mL 0.8389 mL 2.0973 mL
40 mM 0.0629 mL 0.3146 mL 0.6292 mL 1.5730 mL
50 mM 0.0503 mL 0.2517 mL 0.5034 mL 1.2584 mL
60 mM 0.0419 mL 0.2097 mL 0.4195 mL 1.0487 mL
80 mM 0.0315 mL 0.1573 mL 0.3146 mL 0.7865 mL
100 mM 0.0252 mL 0.1258 mL 0.2517 mL 0.6292 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lobaplatin135558-11-1D-19466络铂D19466D 19466ApoptosisCaspaseBcl-2 Familydiastereometric mixture of platinum(II) complexecell cyclecaspaseBaxesophageal squamous cell carcinomaInhibitorinhibitorinhibit

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