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Mag-indo-1/AM 是一种具有细胞渗透性的镁离子荧光指示剂,也可指示钙离子 (Ex=340-390 nm, Em=410-490 nm)。Mag-indo-1/AM 可用于测定细胞内镁离子和钙离子浓度。

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Mag-indo-1/AM Chemical Structure

Mag-indo-1/AM Chemical Structure

CAS No. : 130926-94-2

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of intracellular Ca2+[1]:
1. Incubate 3 mL cell suspension with 10 μM Mag-indo-1/AM for 15 min at 30℃.
2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl2).
3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use.
4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature.
6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope.
7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca2+ bound and Ca2+ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

730.63

Formula

C33H34N2O17

CAS 号
Emission (Em)

490

Excitation (Ex)

340

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

Mag-indo-1/AM 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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产品名称:
Mag-indo-1/AM
目录号:
HY-D1700
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