Mag-Indo-1 tetrapotassium salt 

Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg²⁺ detection^[1].

Mag-Indo-1 tetrapotassium salt can be used to determine the free Mg²⁺ concentration in the cytoplasmic solutions^[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Calibrating solutions containing various concentrations of Mg²⁺ are prepared by mixing different ratios of the two stock solutions: one contains (mM) 150 KCl, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH), and the other contains 100 MgCl₂, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH).
2. The calibration curve is constructed using calibrating solutions containing 1 μM Mag-Indo-1 tetrapotassium salt.
3. The ‘Mg²⁺’ calibrating solution contained (mM): 130 KCl, 4 EDTA and 5 Hepes (pH 7.2 with KOH).
4. The relationship between the background-corrected value of the fluorescence ratio (R) and the Mg²⁺ concentration is fitted with the following theoretical equation.
[Mg]=K (R-R_min)/(R_max-R), where [Mg] is the concentration of free Mg²⁺ ion, R_min is the R value at 0 [Mg²⁺], and R_max is the R value at saturating Mg²⁺. The curve fitting gave R_min= 0.053, R_max= 1.57, and K= 5.1 mM.
Note: the specific curve can refer to the reference.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

594.74

C₂₁H₁₄K₄N₂O₉

132299-21-9

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ding-Hong Yan, et al. Two Kir2.1 channel populations with different sensitivities to Mg(2+) and polyamine block: a model for the cardiac strong inward rectifier K(+) channel. J Physiol. 2005 Mar 15;563(Pt 3):725-44.  [Content Brief]