1. Academic Validation
  2. Impact of knockdown LincRNA-Cox2 on apoptosis of macrophage infected with Bacillus Calmette-Guérin

Impact of knockdown LincRNA-Cox2 on apoptosis of macrophage infected with Bacillus Calmette-Guérin

  • Mol Immunol. 2021 Feb;130:85-95. doi: 10.1016/j.molimm.2020.11.008.
Yanan Xu 1 Jialin Yu 1 Chenjie Ma 1 Zhaoqian Gong 1 Xiaoling Wu 2 Guangcun Deng 3
Affiliations

Affiliations

  • 1 Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, NingXia University, NingXia, Yinchuan, 750021, China; School of Life Science, NingXia University, NingXia, Yinchuan, 750021, China.
  • 2 Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, NingXia University, NingXia, Yinchuan, 750021, China; School of Life Science, NingXia University, NingXia, Yinchuan, 750021, China. Electronic address: wuxiaol@nxu.edu.cn.
  • 3 Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, NingXia University, NingXia, Yinchuan, 750021, China; School of Life Science, NingXia University, NingXia, Yinchuan, 750021, China. Electronic address: dgc@nxu.edu.cn.
Abstract

Mycobacterium tuberculosis (Mtb)-induced Apoptosis of alveolar macrophages plays an important role in the pathogenesis of tuberculosis. Previous studies indicated that massive LncRNAs could deteriorate MTB invasion or latent Infection by regulating macrophage's Apoptosis. However, whether LincRNA-Cox2 is involved in Apoptosis of macrophage infected with Mtb is unclear. In this study, we found Bacillus Calmette-Guerin(BCG)Infection induced cell Apoptosis with a increasing LincRNA-Cox2 expression in RAW264.7 cells. Furthermore, the activation of TLR signal pathway elevated the expression of lincRNA-Cox2. In this regard, we used small interfering RNA to explore the role of LincRNA-Cox2 on regulating Apoptosis of RAW264.7 cells infected with BCG. The results showed that si-LincRNA-Cox2 was capable of increased the expression of apoptosis-associated proteins and accumulation of ROS in BCG-infected RAW264.7 cells. Mechanically, si-LincRNA-Cox2 facilitated BCG-induced macrophage Apoptosis by activating the intrinsic apoptotic pathway as well as increased the genes expression of PERK/eIF2α/CHOP. These results provide novel insights into host-pathogen interactions and highlight the potential role of LincRNA-Cox2 in regulating Apoptosis induced by BCG-infection.

Keywords

Apoptosis; BCG; Endoplasmic reticulum stress; LincRNA-Cox2.

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