Pep2-SVKE 

Pep2-SVKE is an inactive control peptide for pep2-SVKI (HY-P1056). Pep2-SVKE is an inhibitory peptide corresponding to the last 10 amino acids of the C-terminus of the GluR2 AMPA receptor subunit. Pep2-SVKE does not block AMPA-mediated [³H]DA exocytosis. Pep2-SVKE does not bind to GRIP or PICK43 and does not block retention of PICK1 by GST-GluR2 and LTD^{[1][2][3][4][5]}.

Pep2-SVKE (100 μM) 不会阻断多巴胺能 (DA) 神经元的 mGluR-LTD^[1]。

Pep2-SVKE (1 mM) 无法与 PICK 或 GRIP46 结合，也不会抑制活性肽诱导的 EPSC 振幅比 (R+40/-60mV) 的增加^[2] 。

Pep2-SVKE (1 mM) 不会与 GRIP 或 PICK43 结合，也不会改变体细胞 AMPAR 受体的 mEPSC 振幅和 AMPA 和 NMDA 诱导的 [³H]ACh 释放^[2][4]。

Pep2-SVKE (1 μM) 不会改变 [³H]DA 溢流的急剧减少^[3]。

Pep2-SVKE 不会消除 AMPA 受体对环噻嗪的敏感性，而环噻嗪可控制 [³H] 核苷类似物 (NA) 的释放^[4]。

Pep2-SVKE (133 μM) 不会阻断 GST-GluR2 和 LTD 对 PICK1 的保留^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1300.41

C₅₉H₈₉N₁₃O₂₀

1315378-76-7

Tyr-Asn-Val-Tyr-Gly-Ile-Glu-Ser-Val-Lys-Glu

YNVYGIESVKE

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mameli M, et al. Rapid synthesis and synaptic insertion of GluR2 for mGluR-LTD in the ventral tegmental area. Science. 2007 Jul 27;317(5837):530-3.  [Content Brief]

  [2]. Liu SJ, et al. Subunit interaction with PICK and GRIP controls Ca2+ permeability of AMPARs at cerebellar synapses. Nat Neurosci. 2005 Jun;8(6):768-75.  [Content Brief]

  [3]. Grilli M, et al. In vitro exposure to nicotine induces endocytosis of presynaptic AMPA receptors modulating dopamine release in rat nucleus accumbens nerve terminals. Neuropharmacology. 2012 Oct;63(5):916-26.  [Content Brief]

  [4]. Pittaluga A, et al. Trafficking of presynaptic AMPA receptors mediating neurotransmitter release: neuronal selectivity and relationships with sensitivity to cyclothiazide. Neuropharmacology. 2006 Mar;50(3):286-96.  [Content Brief]

  [5]. Daw MI, et al. PDZ proteins interacting with C-terminal GluR2/3 are involved in a PKC-dependent regulation of AMPA receptors at hippocampal synapses. Neuron. 2000 Dec;28(3):873-86.  [Content Brief]