Pyranine hydrate  (Synonyms: HPTS hydrate; Solvent Green 7 hydrate)

Pyranine (HPTS; Solvent Green 7) hydrate is a pH-sensitive fluorescent indicator. Pyranine hydrate acts as a class of fluorescent chemosensor for the Cu⁺ ion. Pyranine hydrate is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm)^[1][2][3].

即使在竞争环境中，Pyranine hydrate 也能区分来自 Cu⁺ 离子的阳离子范围。Pyranine hydrate 对 Cu⁺ 离子表现出快速的荧光响应(t_1/2=1.66 分钟)，微摩尔检测限使其能够检测环境样品中的离子。观察到的 Pyranine hydrate 与 Cu⁺ 之间的络合化学计量为 2:1^[1]。
研究了 pH 敏感的荧光指示剂 Pyranine hydrate，以确定其作为活体角膜和前房探针的有效性。局部给药后，在角膜和前房中达到足够的浓度；可以通过荧光光度法进行数小时的测量。pH 值是通过测量两个激发波长 I463/I404 下的荧光强度比值来计算的，该测量值取决于 pH 值，但与荧光团浓度及其他可能改变强度的变量无关^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

542.40

C₁₆H₉Na₃O₁₁S₃

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33.  [Content Brief]

  [2]. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9.  [Content Brief]

  [3]. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47.  [Content Brief]