Enolase 1/ENO1 蛋白, Human (His)

Alpha-enolase/Enolase 1 Protein, Human (His) expresses in E. coli with a His tag at the N-terminus. Alpha-enolase, a multifunctional protein, is a key glycolytic enzyme in the cytoplasm of prokaryotic and eukaryotic cells^[1].

α-烯醇化酶在多种细胞类型的表面表达，充当纤溶酶原受体，将蛋白水解纤溶酶活性集中在细胞表面。α-烯醇酶的差异表达与多种病理有关，例如癌症、阿尔茨海默氏病和类风湿性关节炎等。α-烯醇酶可被视为多种疾病中病理应激的标志物，发挥多种功能，主要作为纤溶酶原受体^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

P06733 (M1-K434)

2023  [NCBI]

MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

45-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, 1 mM MgSO₄, 20% Glycerol, 5% Trehalose, 5% Mannitol, 0.02% Tween80, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Angels Díaz-Ramos, et al. α-Enolase, a multifunctional protein: its role on pathophysiological situations. J Biomed Biotechnol. 2012;2012:156795.  [Content Brief]