CD8 alpha 蛋白, Rat (HEK293, Fc)

CD8 alpha is an important immune glycoprotein that serves as a coreceptor for MHC class I:peptide complexes, linking T cells to antigens presented by APCs. It interacts with TCR and MHC class I, recruits LCK kinase, and initiates multiple signaling pathways. CD8 alpha Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD8 alpha protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD8 alpha Protein, Rat (HEK293, Fc) is 189 a.a., with molecular weight of 50-56 kDa.

CD8 α 蛋白是一种完整的膜糖蛋白，在免疫反应中发挥着关键作用，在针对外部和内部威胁的反应中发挥多种功能。在 T 细胞中，它的主要功能是作为 MHC I 类分子：肽复合物的辅助受体，同时与 T 细胞受体 (TCR) 和抗原呈递细胞 (APC) 呈递的 MHC I 类蛋白相互作用。这种相互作用促进 Src 激酶 LCK 募集到 TCR-CD3 复合物附近。反过来，LCK 通过磷酸化不同的底物启动各种细胞内信号传导途径，最终导致淋巴因子的产生、运动、粘附和细胞毒性 T 淋巴细胞 (CTL) 的激活。这种机制使 CTL 能够识别并消除受感染的细胞和肿瘤细胞。在 NK 细胞中，细胞表面 CD8A 同二聚体的存在提供了一种生存机制，允许多个靶细胞的接合和裂解。CD8A 同二聚体分子还有助于活化淋巴细胞的存活和分化为记忆 CD8 T 细胞。CD8 α 在细胞表面与 CD8B 形成二硫键连接的异二聚体，并且在各种细胞类型（包括 NK 细胞和外周血 T 淋巴细胞）中形成同二聚体。此外，它还与 MHC I 类 HLA-A/B2M 二聚体相互作用，并以锌依赖性方式与 LCK 结合。

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 2.584 μg/mL, corresponding to a specific activity is 387.0 units/mg.

Rat

HEK293

C-hFc

P07725 (Q27-Y189)

24930  [NCBI]

QLQLSPKKVDAEIGQEVKLTCEVLRDTSQGCSWLFRNSSSELLQPTFIIYVSSSRSKLNDILDPNLFSARKENNKYILTLSKFSTKNQGYYFCSITSNSVMYFSPLVPVFQKVNSIITKPVTRAPTPVPPPTGTPRPLRPEACRPGASGSVEGMGLGFACDIY

Approximately 50-56 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.