CSK 蛋白, Mouse (sf9, His-GST)

CSK protein is a non-receptor tyrosine protein kinase that regulates cell growth, differentiation, migration and immune responses by phosphorylating Src family kinases (SFK) (such as LCK, SRC, HCK, FYN, LYN, CSK or YES1) . This phosphorylation induces SFK to form an inactive conformation, thereby inhibiting signaling by receptors such as T cell receptor (TCR) and B cell receptor (BCR). CSK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CSK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CSK Protein, Mouse (sf9, His-GST) is 450 a.a., with molecular weight of ~65 kDa.

CSK 蛋白是一种非受体酪氨酸蛋白激酶，在细胞生长、分化、迁移和免疫反应的复杂调节中发挥着关键作用。它通过磷酸化位于 Src 家族激酶 (SFK)（例如 LCK、SRC、HCK、FYN、LYN、CSK 或 YES1）C 末端尾部的酪氨酸残基来实现此目的。这种磷酸化事件诱导 SFK 的磷酸酪氨酸尾部和 SH2 结构域之间的分子内相互作用，从而产生非活性构象。为了抑制 SFK，CSK 通过与邻近的跨膜蛋白或衔接蛋白结合而被募集到质膜上。CSK 通过磷酸化 FYN 或 LCK 等多种正效应子并将其维持在非活性状态，从而在抑制各种表面受体（包括 T 细胞受体 (TCR) 和 B 细胞受体 (BCR)）信号传导方面发挥着至关重要的作用，从而调节关键的细胞反应。

Mouse

Sf9 insect cells

N-His;N-GST

P41241 (M1-L450)

12988  [NCBI]

MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAATRPTFLQLREQLEHIKTHELHL

Approximately 65 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.