DKK-1 蛋白, Human (HEK293, His)

Dkk-1 Protein, Human (HEK293) is involved in embryonic development through its inhibition of the Wnt signaling pathway.

人类 Dickkopf 相关蛋白-1 是 dickopf 家族的成员。它是一种具有两个富含半胱氨酸区域的分泌蛋白，通过抑制 Wnt 信号通路参与胚胎发育。Dickkopf WNT 信号通路抑制剂 1 (Dkk1) 是一种蛋白质编码基因，作用于前内脏内胚层^[1][2]。经证明，DKK1 可通过减少 β-catenin 和增加 OCT4 表达来拮抗 Wnt/β-catenin 通路^[3]。

1. The ED₅₀ is <4 μg/mL as measured in stimulation of alkaline phosphatase activity using CCl-226 cells.
2. Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by  C3H10T1/2 cells. The ED₅₀ for this effect is approximately 0.1-0.8 μg/mL  in the presence of 10 ng/mL of mouse Wnt3a.

Human

HEK293

C-His

O94907 (T32-R265)

22943  [NCBI]

TLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQR

Approximately 35-50 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Schneider VA, et al. Spatially distinct head and heart inducers within the Xenopus organizer region. Curr Biol. 9: 800–809.

  [2]. Mukhopadhyay M, et al. Dickkopf1 is required for embryonic head induction and limb morphogenesis in the mouse. Developmental Cell. 1 (3): 423–34.  [Content Brief]

  [3]. Ou L, et al. Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo. Molecular Medicine Reports. 13 (1): 720–30.  [Content Brief]