1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. EGF
  5. EGF 蛋白, Mouse

EGF 蛋白, Mouse

目录号: HY-P7067
COA 产品使用指南

EGF 蛋白, Mouse是一种小分子多肽,可刺激表皮和一些上皮组织的增殖。

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规格 价格 是否有货 数量
10 μg ¥350 In-stock
50 μg ¥900 In-stock
100 μg ¥1600 询价
250 μg   询价  

* Please select Quantity before adding items.

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  • 生物活性

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  • 产品资料

  • 参考文献

描述

EGF Protein, Mouse is a small polypeptide that stimulates the proliferation of the epidermis and several epithelial tissues.

研究背景

Epidermal growth factor (EGF) binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis[1]. Epidermal growth factor (EGF) is initially synthesized as a large precursor of 1217 amino acids that is glycosylated and can be secreted by cells. Epidermal growth factor (EGF) mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney[2].

生物活性

The ED50 is <0.1ng/mL as measured by BALB/c 3T3 cells, corresponding to a specific activity of >1.0 × 107 units/mg.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P01132 (N977-R1029)

基因 ID

13645  [NCBI]

同用名
rMuEGF; Pro-epidermal growth factor; Urogastrone
氨基酸序列

MNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR

分子量

Approximately 6.2 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

EGF Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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