1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. EGF
  5. EGF 蛋白, Human

EGF 蛋白, Human

目录号: HY-P7109
COA 产品使用指南

EGF 蛋白, Human是一种重要的表皮生长因子,能够刺激表皮细胞增殖,可应用于伤口愈合的研究。

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规格 价格 是否有货 数量
10 μg ¥500 In-stock
50 μg ¥750 In-stock
100 μg ¥1050 询价
250 μg   询价  

* Please select Quantity before adding items.

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描述

EGF Protein, Human is a potent epidermal growth factor, stimulates the proliferation of epidermal cells and is used in wound healing applications.

研究背景

Recombinant Human Epidermal Growth Factor is chemically identical to the natural material, exhibits full biological activity and is ued in wound healing applications[1]. Recombinant Human Epidermal Growth Factor (rhEGF) stimulates proliferation of the fibroblast BALB/c3T3 cell line. Recombinant Human Epidermal Growth Factor released from hydrogels keeps its bioactivity, induces EGF receptor expression, causes proliferating cell nuclear antigen and shows therapeutic potential in enhancing diabetic wound healing[2].

生物活性

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P01133 (N971-R1023)

基因 ID

1950  [NCBI]

中文名
重组人表皮生长因子
同用名
rHuEGF; Pro-epidermal growth factor; Urogastrone
氨基酸序列

MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

分子量

Approximately 11 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200 mM NaCl buffer.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

EGF Protein, Human 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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