1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. EGF
  5. EGF 蛋白, Human

EGF 蛋白, Human

目录号: HY-P7109
COA 产品使用指南

EGF 蛋白, Human是一种重要的表皮生长因子,能够刺激表皮细胞增殖,可应用于伤口愈合的研究。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥300 In-stock
10 μg ¥570 In-stock
50 μg ¥1080 In-stock
100 μg ¥1620 In-stock
500 μg ¥3240 In-stock
1 mg ¥5000 In-stock
> 100 μg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

EGF Protein, Human is a potent epidermal growth factor, stimulates the proliferation of epidermal cells and is used in wound healing applications.

研究背景

重组人表皮生长因子的化学成分与天然材料相同,具有完整的生物活性,可用于伤口愈合应用[1]。重组人表皮生长因子 (rhEGF) 刺激成纤维细胞 BALB/c3T3 细胞系的增殖。从水凝胶中释放的重组人表皮生长因子可保持其生物活性,诱导 EGF 受体表达,引起细胞核抗原增殖,并在促进糖尿病伤口愈合方面显示出治疗潜力[2]

生物活性

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is <300 pg/mL.

  • Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is 279.4 pg/mL, corresponding to a specific activity is 3.5791×106 units/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P01133-1 (N971-R1023)

基因 ID
蛋白结构
N-term
EGF (N971-R1023)
Accession # P01133
C-term
中文名
重组人表皮生长因子
同用名
rHuEGF; Pro-epidermal growth factor; Urogastrone
氨基酸序列

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

分子量

Approximately 7-10 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 10 mM Phosphate buffer, pH 7.0, 200 mM NaCl buffer or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Tris, 200 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

EGF Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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