1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. EGF
  5. EGF 蛋白, Human

EGF 蛋白在体内和体外环境中均可作为多种表皮和上皮组织生长的有效刺激剂,同时促进细胞培养中特定成纤维细胞的增殖。这种多面蛋白还充当镁刺激激素,通过 EGFR 的参与和镁通道 TRPM6 的激活驱动肾远曲小管中的镁重吸收。EGF 蛋白, Human 是重组的 EGF 蛋白,由 E. coli 表达,不带标签。

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2 μg ¥300 In-stock
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MCE 顾客使用本产品发表的科研文献

  • 客户验证

MCE 生物验证

IP
    Customer Validation
    EGF (100 ng/mL; 10 h) increases the interaction between MYG1 and HSP90 in 293 T cells instead of LoVo cells with KRAS mutation.
    • 生物活性

    • 技术参数

    • 产品性质

    • 产品资料

    • 参考文献

    描述

    EGF proteins act as potent stimulators of growth in a variety of epidermal and epithelial tissues in both in vivo and in vitro settings, while promoting the proliferation of specific fibroblasts in cell culture. This multifaceted protein also acts as a magnesium stimulating hormone, driving magnesium reabsorption in the renal distal tubule through engagement of EGFR and activation of the magnesium channel TRPM6. EGF Protein, Human is the recombinant human-derived EGF protein, expressed by E. coli , with tag free.

    研究背景

    EGF 蛋白在体内和体外环境中成为多种表皮和上皮组织生长的有效刺激剂,同时促进细胞培养中特定成纤维细胞的增殖。这种多面蛋白还充当促镁激素,通过 EGFR 的参与和镁通道 TRPM6 的激活驱动肾远曲小管中的镁重吸收。值得注意的是,EGF 蛋白具有在体外诱导池塘蜗牛 Lymnaea stagnalis 运动神经元神经突生长的能力。其分子相互作用包括与 EGFR 结合,从而促进 EGFR 二聚化,以及与 RHBDF2 相互作用,表明参与多种细胞过程。此外,EGF 蛋白与 RHBDF1 结合,可能影响 EGF 在内质网中的保留,并通过内质网相关降解 (ERAD) 调节其降解。

    体外研究
    (In Vitro)

    EGF Protein, Human (0-500 ng/mL, 72 h) 促进 NIH 3T3 细胞增殖[3]

    体内研究
    (In Vivo)

    EGF 蛋白 (Human) (10 μg/g, 乳膏, 单次剂量, 持续 7 天)在全层皮肤创口的大鼠中可以减少炎症浸润,并促使伤口再上皮化[1]
    EGF 蛋白 (Human) (50 μg/g,乳膏,每 24 小时,连用 7 天) 可显著刺激有背部厚创伤猪的再上皮细胞形成,最好与蛋白酶抑制剂联合使用[1]
    EGF 蛋白 (Human) (30 μg/kg, 皮下注射, 每天注射, 持续 3 周) 可以减轻戈廷根迷你猪中由硬化疗法 (食道的外科扩张) 导致的食道损伤[1]

    生物活性

    Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is <300 pg/mL.

    • Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is 53.08 pg/mL, corresponding to a specific activity is 1.88×107 units/mg.
    种属

    Human

    表达系统

    E. coli

    标签

    Tag Free

    蛋白编号

    P01133-1 (N971-R1023)

    基因 ID
    蛋白结构
    N-term
    EGF (N971-R1023)
    Accession # P01133
    C-term
    蛋白长度

    Full Length of EGF

    中文名
    表皮生长因子蛋白
    同用名
    rHuEGF; Pro-epidermal growth factor; Urogastrone
    氨基酸序列

    NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

    预测分子量
    5.6 kDa
    分子量

    Approximately 7 kDa under reducing conditions.

    纯度
    • Greater than 95% as determined by reducing SDS-PAGE.
    性状

    无菌粉末

    组分

    Lyophilized from a 0.2 μm filtered solution of 10 mM Phosphate buffer, pH 7.0, 200 mM NaCl or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Tris, 200 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

    内毒素含量

    <1 EU/μg, determined by LAL method.

    复溶方法

    It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

    保存条件 & 期限

    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

    运输条件

    Room temperature in continental US; may vary elsewhere.

    文件资料
    参考文献

    EGF Protein, Human 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • 复溶计算器

    • 重组蛋白稀释计算器

    • 比活力计算器

    The reconstitution calculator equation

    Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

    Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
    = ÷

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2

    The specific activity calculator equation

    Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

    Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
    Unit/mg = 106 ÷ ng/mL

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