EGF 蛋白, Human

EGF proteins act as potent stimulators of growth in a variety of epidermal and epithelial tissues in both in vivo and in vitro settings, while promoting the proliferation of specific fibroblasts in cell culture. This multifaceted protein also acts as a magnesium stimulating hormone, driving magnesium reabsorption in the renal distal tubule through engagement of EGFR and activation of the magnesium channel TRPM6. EGF Protein, Human is the recombinant human-derived EGF protein, expressed by E. coli , with tag free.

EGF 蛋白在体内和体外环境中成为多种表皮和上皮组织生长的有效刺激剂，同时促进细胞培养中特定成纤维细胞的增殖。这种多面蛋白还充当促镁激素，通过 EGFR 的参与和镁通道 TRPM6 的激活驱动肾远曲小管中的镁重吸收。值得注意的是，EGF 蛋白具有在体外诱导池塘蜗牛 Lymnaea stagnalis 运动神经元神经突生长的能力。其分子相互作用包括与 EGFR 结合，从而促进 EGFR 二聚化，以及与 RHBDF2 相互作用，表明参与多种细胞过程。此外，EGF 蛋白与 RHBDF1 结合，可能影响 EGF 在内质网中的保留，并通过内质网相关降解 (ERAD) 调节其降解。

EGF Protein, Human (0-500 ng/mL, 72 h) 促进 NIH 3T3 细胞增殖^[3]。

EGF 蛋白 (Human) (10 μg/g, 乳膏, 单次剂量, 持续 7 天）在全层皮肤创口的大鼠中可以减少炎症浸润，并促使伤口再上皮化^[1]。
EGF 蛋白 (Human) (50 μg/g，乳膏，每 24 小时，连用 7 天) 可显著刺激有背部厚创伤猪的再上皮细胞形成，最好与蛋白酶抑制剂联合使用^[1]。
EGF 蛋白 (Human) (30 μg/kg, 皮下注射, 每天注射, 持续 3 周) 可以减轻戈廷根迷你猪中由硬化疗法 (食道的外科扩张) 导致的食道损伤^[1]。

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is <300 pg/mL.

• 
  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED₅₀ for this effect is 53.08 pg/mL, corresponding to a specific activity is 1.88×10⁷ units/mg.

Human

E. coli

Tag Free

P01133-1 (N971-R1023)

1950  [NCBI]

Full Length of EGF

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Approximately 7 kDa under reducing conditions.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Phosphate buffer, pH 7.0, 200 mM NaCl or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Tris, 200 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wong WR, et al. Applications, and efficient large-scale production, of recombinant human epidermal growth factor. Biotechnol Genet Eng Rev. 2001;18:51-71.  [Content Brief]

  [2]. Lao G, et al. Controlled release of epidermal growth factor from hydrogels accelerates wound healing in diabetic rats. J Am Podiatr Med Assoc. 2012 Mar-Apr;102(2):89-98.  [Content Brief]

  [3]. Pouranvari S, et al., Cloning, Expression, and Cost Effective Purification of Authentic Human Epidermal Growth Factor With High Activity. Iran Red Crescent Med J. 2016 Mar 20;18(3):e24966.  [Content Brief]