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Galectin-1/LGALS1 蛋白, Human (His)

目录号: HY-P70273
COA 产品使用指南

Galectin-1/LGALS1 是一种凝集素,可结合 β-半乳糖苷和复合碳水化合物并调节细胞凋亡、增殖和分化。它抑制 CD45 磷酸酶活性,阻断 Lyn 激酶去磷酸化,并诱导 T 细胞凋亡。Galectin-1/LGALS1 蛋白, Human (His) 是重组的 Galectin LGALS1 蛋白,由 E. coli 表达,带有 C-6*His 标签。Galectin-1/LGALS1 蛋白, Human (His) 全长 134 个氨基酸,分子量约为 ~15.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥500 In-stock
50 μg ¥1500 In-stock
100 μg ¥2550 In-stock
> 100 μg   询价  

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描述

Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human (His) is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Galectin-1/LGALS1 Protein, Human (His) is 134 a.a., with molecular weight of ~15.0 kDa.

研究背景

Galectin-1/LGALS1 蛋白作为一种凝集素,能够结合 β-半乳糖苷和多种复杂碳水化合物。它在细胞凋亡、细胞增殖和细胞分化的调节中起着关键作用。Galectin-1/LGALS1 通过抑制 CD45 的蛋白磷酸酶活性发挥作用,从而阻碍 Lyn 激酶的去磷酸化。此外,它还是 T 细胞凋亡的有效诱导剂。Galectin-1/LGALS1 以同二聚体形式存在,与多种细胞实体形成相互作用,包括 CD2、CD3、CD4、CD6、CD7、CD43、ALCAM 和 CD45。它还结合 LGALS3BP、层粘连蛋白(通过聚-N-乙酰基乳糖胺)和 SUSD2。值得注意的是,Galectin-1/LGALS1 与货物受体 TMED10 相互作用,促进从细胞质易位到内质网-高尔基中间室 (ERGIC) 和随后的分泌。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P09382 (A2-D135)

基因 ID
蛋白结构
N-term
LGALS1 (A2-D135)
Accession # P09382
6*His
C-term
同用名
rHuGalectin-1/LGALS1, His; Galectin-1; Gal-1; 14 kDa Laminin-Binding Protein; HLBP14; 14 kDa Lectin; Beta-Galactoside-Binding Lectin L-14-I; Galaptin; HBL; HPL; Lactose-Binding Lectin 1; Lectin Galactoside-Binding Soluble 1; Putative MAPK-Activating Protein PM12; S-Lac Lectin 1; LGALS1
氨基酸序列

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

分子量

Approximately 15.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8% Trehalose, 4% Mannitol, 50 mM NaCl, 1 mM TCEP, 0.05% Tween 80, pH 4.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Galectin-1/LGALS1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Galectin-1/LGALS1 Protein, Human (His)
目录号:
HY-P70273
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