IL-12R beta 1 蛋白, Human (HEK293, His)

IL-12R beta 1 protein is an IL-12 cytokine surface receptor that activates the Jak-Stat signaling cascade pathway and is involved in IL-12-mediated immune regulation. IL-12R beta 1 Protein, Human (HEK293, His) is expressed by HEK 293 cells with a transmembrane region (W546-L570) and a C-terminal 6*His tag^[1].

IL-12 受体是与 IL-12 结合的 I 型细胞因子受体。它主要在 NK 和 T 细胞上表达，由 β1 和 β2 亚基组成，是 gp130 细胞因子受体超家族的成员。IL-12R beta 1（缩写为 IL-12R1 或 IL-12Rβ1）是 IL-12 受体的一个亚基。IL-12R beta 1，也称为CD212（分化簇212），是其人类基因的名称。它编码属于造血素受体超家族的 I 型跨膜蛋白。IL-12Rβ1 可形成二硫键连接的寡聚体，这是 IL-12 结合活性所必需的，并以低亲和力与 IL-12 结合。同时，IL-12Rβ1 蛋白可以与 IL-12Rβ2 蛋白共表达，从而形成高亲和力的 IL-12 结合位点并重建 IL-12 依赖性信号传导^[1]。
IL-12 与 IL-12 受体结合后，直接与 IL-12Rβ1 相互作用的细胞质蛋白 TYK2 和与 IL-12Rβ2 相互作用的 JAK2 被酪氨酸磷酸化。磷酸化的 TYK2 和 JAK2 是随后的酪氨酸磷酸化和 STAT4 激活所必需的，STAT4 与 IL-12Rβ2 结合。STAT4 是一种转录因子，随后同二聚化、易位到细胞核并与其靶 DNA 结合，激活 IFN-γ 和其他靶基因的转录。IL-12Rβ1 还与 IL23R 结合形成 IL-23 受体，参与 IL-23 信号转导。它可能通过激活 Jak-Stat 信号级联在 IL-23 信号传导中发挥作用。IL-12Rβ1 在淋巴细胞表面以低强度组成型表型表达，可通过 T 细胞激活或各种白细胞介素（如 IL-2、IL-7 和 IL-15）刺激而高度上调。它也在树突细胞上表达。该基因缺乏表达会导致严重分枝杆菌和沙门氏菌感染患者的免疫缺陷^[2]。

IL-12Rβ1-deficient patients are able to develop Th1 T cells, and most Th1 T cell clones (TCCs) are able to respond specifically and consistently to IL-12 by increasing IFN-γ production^[1].
IL-12Rβ1 can be expressed on human monocyte-derived dendritic cells (DCs) as well as T cells (TCs) and Con A parent cells, and IL-12Rβ1 is expressed at higher levels on monocyte-derived DCs than on TCs and Con A parent cells. In contrast, little or no IL-12Rβ1 expression is observed in monocytes. IL-12Rβ1 binding to IL-12 induces tyrosine phosphorylation of a variety of intracellular proteins in DCs^[3].

Human

HEK293

C-6*His

P42701 (C24-E540)

3594  [NCBI]

CRTSECCFQDPPYPDADSGSASGPRDLRCYRISSDRYECSWQYEGPTAGVSHFLRCCLSSGRCCYFAAGSATRLQFSDQAGVSVLYTVTLWVESWARNQTEKSPEVTLQLYNSVKYEPPLGDIKVSKLAGQLRMEWETPDNQVGAEVQFRHRTPSSPWKLGDCGPQDDDTESCLCPLEMNVAQEFQLRRRQLGSQGSSWSKWSSPVCVPPENPPQPQVRFSVEQLGQDGRRRLTLKEQPTQLELPEGCQGLAPGTEVTYRLQLHMLSCPCKAKATRTLHLGKMPYLSGAAYNVAVISSNQFGPGLNQTWHIPADTHTEPVALNISVGTNGTTMYWPARAQSMTYCIEWQPVGQDGGLATCSLTAPQDPDPAGMATYSWSRESGAMGQEKCYYITIFASAHPEKLTLWSTVLSTYHFGGNASAAGTPHHVSVKNHSLDSVSVDWAPSLLSTCPGVLKEYVVRCRDEDSKQVSEHPVQPTETQVTLSGLRAGVAYTVQVRADTAWLRGVWSQPQRFSIE

80-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. C E Verhagen, et al. Residual type 1 immunity in patients genetically deficient for interleukin 12 receptor beta1 (IL-12Rbeta1): evidence for an IL-12Rbeta1-independent pathway of IL-12 responsiveness in human T cells. J Exp Med. 2000 Aug 21;192(4):517-28.  [Content Brief]

  [2]. Esther van de Vosse, et al. IL-12Rβ1 deficiency: mutation update and description of the IL12RB1 variation database. Hum Mutat. 2013 Oct;34(10):1329-39.  [Content Brief]

  [3]. H Nagayama, et al. IL-12 responsiveness and expression of IL-12 receptor in human peripheral blood monocyte-derived dendritic cells. J Immunol. 2000 Jul 1;165(1):59-66.  [Content Brief]