1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-23
  5. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer 蛋白, Human (HEK293, His)

IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer 蛋白, Human (HEK293, His)

目录号: HY-P73193
COA 产品使用指南

IL-23 与 IL12B 共同构成促炎细胞因子 IL-23,在先天免疫和适应性免疫中至关重要。IL-23 由树突状细胞或巨噬细胞等抗原呈递细胞释放,与 IL12RB1 和 IL23R 结合,激活 JAK2TYK2。该级联磷酸化 STAT3 和 STAT4,激活 p38 MAPK 或 NF-kappa-B 等通路,诱导促炎细胞因子。IL-23 有助于细胞内细菌清除并支持 T 辅助细胞 17 细胞(包括 IL-17 产生细胞)的扩增。二硫键连接的 IL-23 与 IL12B 和 IL23R 相互作用,招募 IL12RB1。IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer 蛋白, Human (HEK293, His) 是重组的蛋白二聚体复合物,包含 IL-23 α (170a.a) 和 IL-12 β (306a.a) 异二聚体蛋白,由 HEK293 表达,带有 C-6*His, C-His 标签。IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer 蛋白, Human (HEK293, His),分子量约为 ~55.8 kDa。

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描述

IL-23, collaborating with IL12B, constitutes the pro-inflammatory cytokine IL-23, crucial in innate and adaptive immunity. Released by antigen-presenting cells like dendritic cells or macrophages, IL-23 binds to IL12RB1 and IL23R, activating JAK2 and TYK2. This cascade phosphorylates STAT3 and STAT4, activating pathways like p38 MAPK or NF-kappa-B, inducing pro-inflammatory cytokines. IL-23 contributes to intracellular bacterial clearance and supports the expansion of T-helper 17 cells, including IL-17 producers. The disulfide-linked IL-23 interacts with IL12B and IL23R, recruiting IL12RB1. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived IL-23 alpha, expressed by HEK293, with C-6*His, C-His labeled tag. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (HEK293, His), has molecular weight of ~55.8 kDa.

研究背景

IL-23 与 IL12B 协同形成促炎细胞因子 IL-23,在先天免疫和适应性免疫中发挥多种作用。IL-23 由树突状细胞或巨噬细胞等抗原呈递细胞释放,与包含 IL12RB1 和 IL23R 的异二聚体受体复合物结合,启动涉及 JAK2TYK2 激活的级联反应。这些激酶磷酸化受体,为随后的 STAT3 和 STAT4 磷酸化创建对接位点。该过程激活多种途径,包括 p38 MAPK 或 NF-kappa-B,促进促炎细胞因子的产生,例如 IL-17A/IL17A。此外,IL-23 积极参与细胞内细菌的早期有效清除。值得注意的是,IL-23 促进 T 辅助细胞 17 细胞的扩增和存活,T 辅助细胞 17 是一种以产生 IL-17CD4 阳性辅助 T 细胞亚群,以及其他产生 IL-17 的细胞。IL-23 与 IL12B 的异二聚体(称为白细胞介素 IL-23)是二硫键连接的。此外,IL-23 与 IL23R 相互作用,促进 IL12RB1 的募集。

生物活性

Measured by its ability to induce IL17 secretion by mouse splenocytes and the ED50 is 0.2-15ng/mL.

  • Measured by its ability to induce IL-17 secretion by CTLL-2 cells.The ED50 for this effect is 14.92 ng/mL, corresponding to a specific activity is 6.7×104 U/mg.
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q9NPF7 (R20-P189)&P29460 (I23-S328)

基因 ID
同用名
IL-23 p19/IL-12 p40; IL23; IL-23A; Interleukin 23; SGRF
氨基酸序列

IL-23A:
RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP
IL-12B:
IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS

分子量

Approximately 55.8 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (HEK293, His)
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HY-P73193
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