IL-6 蛋白, Mouse

IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse is a recombinant IL-6 protein expressed by E. coli without a tag^[1][2][3].

IL-6 是由免疫细胞 (如 T 细胞、B 细胞、巨噬细胞) 及非免疫细胞 (如成纤维细胞、内皮细胞、肝细胞) 分泌的多功能细胞因子。IL-6 通过与受体结合传递信号。IL-6 是应对感染和组织损伤时快速短暂产生的细胞因子，通过刺激急性期反应、造血和免疫反应参与宿主防御，其失调可引发慢性炎症和自身免疫病。IL-6 在炎症初始阶段经血液运输至肝脏诱导多种急性期蛋白合成，还参与血清铁、锌水平调节及血小板释放，其在免疫应答中可促进 Th17 分化、抑制 Treg 分化，影响免疫耐受^[1][2][3]。

IL-6 蛋白 (Mouse; 25 ng/mL; 24 h) 在原代小鼠软骨细胞中可促进炎症因子 MCP-1、MMP-3、CCL5、CXCL12 产生和肥大标志物 COL10A1、RUNX2 表达。机制涉及对 JAK2/STAT3 通路的激活^[3]。

IL-6 蛋白 (Mouse; 0.3 μg/kg; 关节内注射; 单次) 在大鼠中可导致脊髓过度兴奋^[3]。

1.The ED₅₀ is <0.02 ng/mL as measured by murine 7TD1 cells, corresponding to a specific activity of >5 × 10⁷ units/mg.
2. Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-6 Protein is immobilized at 5 μg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse IL-6 R alpha. The ED₅₀ for this effect is 20-156 ng/mL.

• 
  Immobilized Mouse IL-6, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 117.5 ng/mL.

Mouse

E. coli

Tag Free

P08505 (F25-T211)

16193  [NCBI]

Full Length of Mature Protein

FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT

Approximately 21.7 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 200 mM NaCl, 500 mM arginine, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Biotechniques. 1992 Apr;12(4):558-63.  [Content Brief]

  [2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295.  [Content Brief]

  [3]. Mima Z, et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. Int Immunopharmacol. 2022 Dec;113(Pt A):109340.  [Content Brief]