MCP-4/CCL13 蛋白, Human

MCP-4/CCL13 Protein, Human is a CC family chemokine with chemotactic effects on basophils, monocytes, macrophages, immature dendritic cells and T cells. CCL13 binds to CCR1, CCR2, CCR3, CCR5, and CCR11 chemokine receptors and is able to induce key immunomodulatory responses through its effects on regulatory muscle, epithelial, and endothelial cells. MCP-4/CCL13 Protein, Human is a recombinant human MCP-4/CCL13 (Q24-T98) protein expressed by E.coli^[1][2].

CCL13又称单核细胞趋化蛋白MCP-4，是属于CC趋化因子家族的小细胞因子，位于人类17号染色体上。在氨基酸水平上，人类CCL13与人类CCL2有65%的同一性。CCL13 通过与细胞表面 G 蛋白连接的趋化因子受体（例如 CCR1、CCR2、CCR3、CCR5 和 CCR11）结合，可以充当单核细胞、巨噬细胞、T 淋巴细胞、未成熟 DC、嗜酸性粒细胞和嗜碱性粒细胞的趋化因子。CCL13 还会诱导嗜碱性粒细胞释放组胺和嗜酸性粒细胞脱粒，并导致内皮细胞、上皮细胞和肌肉细胞表达粘附分子并产生促炎细胞因子^[1]。CCL13在过敏性和非过敏性炎症两侧的白细胞积聚中发挥作用。研究表明，CCL13 在哮喘和过敏性鼻炎中表达上调，并且与患者气道中募集的单核细胞/巨噬细胞和嗜酸性粒细胞的数量有关。它可能参与动脉粥样硬化疾病期间单核细胞向动脉壁的募集，并在长期暴露于外源病原体的组织中吸引单核细胞方面发挥作用。ccl13 还可能通过促进细胞凋亡和耐药性来促进肿瘤细胞耐药性的发展^[2]。

1. Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 10-100 ng/mL.
2. Measured by its ability to chemoattract THP-1 cells. The ED₅₀ this effect is 18.2 ng/mL, corresponding to a specific activity is 5.49×10⁴ units/mg.

• 
  Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 18.2 ng/mL, corresponding to a specific activity is 5.49×10⁴ units/mg.

Human

E. coli

Tag Free

Q99616 (Q24-T98)

6357  [NCBI]

QPDALNVPSTCCFTFSSKKISLQRLKSYVITTSRCPQKAVIFRTKLGKEICADPKEKWVQNYMKHLGRKAHTLKT

Approximately 12.59 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 130 mM NaCl or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Mendez-Enriquez E, et al. The multiple faces of CCL13 in immunity and inflammation. Inflammopharmacology. 2013 Dec;21(6):397-406.  [Content Brief]

  [2]. Shaoping She, et al. Functional Roles of Chemokine Receptor CCR2 and Its Ligands in Liver Disease. Front Immunol. 2022 Feb 25;13:812431.  [Content Brief]