MUSK 蛋白, Human (P.pastoris, His)

MUSK protein is a receptor tyrosine kinase that plays a crucial role in the formation and maintenance of the neuromuscular junction (NMJ). After LRP4 recruits AGRIN, MUSK phosphorylates and activates, affecting gene expression, coordinating actin cytoskeletal reorganization, and clustering acetylcholine receptors (AChR) in the postsynaptic membrane. MUSK Protein, Human (P.pastoris, His) is the recombinant human-derived MUSK protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of MUSK Protein, Human (P.pastoris, His) is 472 a.a., with molecular weight of ~66 kDa.

MUSK 蛋白是一种受体酪氨酸激酶，在神经肌肉接头 (NMJ)（连接运动神经元和骨骼肌的突触）的形成和维护中发挥着关键作用。当 LRP4 将 AGRIN 招募到 MUSK 信号复合体后，MUSK 会发生磷酸化和激活，通过影响突触下核中的基因表达、协调肌动蛋白细胞骨架重组以及在突触后膜中聚集乙酰胆碱受体 (AChR) 来调节 NMJ 形成。由 MUSK 激活的 ABL1 和 Src 家族激酶可能进一步调节 AChR 磷酸化和聚类。由 MUSK、DVL1 和 PAK1 形成的三元复合物对于 AChR 聚类调控至关重要。此外，MUSK 通过 FNTA 正向调节 Rho 家族 GTPases，介导 FNTA 的磷酸化并促进 RAC1 的激活，RAC1 是肌动蛋白细胞骨架和基因表达的关键调节因子。DNAJA3 作用于 MUSK 下游，是 MUSK 信号通路中的另一个效应子。除了神经肌肉接头之外，麝香还可能有助于中枢神经系统内的胆碱能反应、突触可塑性和记忆形成。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

O15146-2 (L24-I495)

4593  [NCBI]

LPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDI

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.2 μm filtered solution in 20 mM Tris-HC1, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.