Neuroserpin 蛋白, Mouse (HEK293, His)

Neuroserpin is a serine protease inhibitor that significantly inhibits plasminogen activator and plasmin without affecting thrombin. In addition to their role in protease inhibition, neuroserine proteases contribute to synaptic plasticity, affecting synaptic connections in the adult nervous system. Neuroserpin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Neuroserpin protein, expressed by HEK293 , with C-His labeled tag. The total length of Neuroserpin Protein, Mouse (HEK293, His) is 394 a.a., with molecular weight of 45-50 kDa.

Neuroserpin 是一种丝氨酸蛋白酶抑制剂，在抑制纤溶酶原激活剂和纤溶酶方面发挥着至关重要的作用，但对凝血酶没有抑制作用。除了作为蛋白酶抑制剂的作用外，它还参与突触连接的形成或重组，有助于成人神经系统的突触可塑性。此外，神经丝氨酸蛋白酶抑制剂在保护神经元免受组织型纤溶酶原激活剂介导的细胞损伤方面具有保护作用。这些功能强调了神经丝氨酸蛋白酶抑制剂在调节蛋白水解活性和维持复杂的突触过程网络中神经元完整性方面的重要性。

Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ for this effect is 0.894 μg/mL, corresponding to a specific activity is 1.118×10^[3] units/mg.

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  Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ for this effect is 0.894 μg/mL, corresponding to a specific activity is 1.118×10³ units/mg.

Mouse

HEK293

C-His

O35684 (T17-L410)

20713  [NCBI]

TGATFPDETITEWSVNMYNHLRGTGEDENILFSPLSIALAMGMMELGAQGSTRKEIRHSMGYEGLKGGEEFSFLRDFSNMASAEENQYVMKLANSLFVQNGFHVNEEFLQMLKMYFNAEVNHVDFSQNVAVANSINKWVENYTNSLLKDLVSPEDFDGVTNLALINAVYFKGNWKSQFRPENTRTFSFTKDDESEVQIPMMYQQGEFYYGEFSDGSNEAGGIYQVLEIPYEGDEISMMLALSRQEVPLATLEPLLKAQLIEEWANSVKKQKVEVYLPRFTVEQEIDLKDILKALGVTEIFIKDANLTAMSDKKELFLSKAVHKSCIEVNEEGSEAAAASGMIAISRMAVLYPQVIVDHPFLYLIRNRKSGIILFMGRVMNPETMNTSGHDFEEL

Approximately 45-58 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.