OGDC-E 蛋白, Mouse (His-SUMO)

The OGDC-E Protein, a vital component of the 2-oxoglutarate dehydrogenase complex, drives the conversion of 2-oxoglutarate to succinyl-CoA in the mitochondrion, a pivotal step in the Krebs cycle. It employs thiamine diphosphate for decarboxylation, connecting to the E2 enzyme and participating in nuclear histone succinylation with KAT2A. OGDC-E Protein, Mouse (His-SUMO) is the recombinant mouse-derived OGDC-E protein, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of OGDC-E Protein, Mouse (His-SUMO) is 329 a.a., with molecular weight of ~54 kDa.

OGDC-E 蛋白是 2-酮戊二酸脱氢酶复合物 (OGDHC) 的组成部分，特别是 E1o 组成部分。它在 OGDHC 内将 2-酮戊二酸转化为琥珀酰辅酶 A 和 CO(2) 的第一步和限速步骤中发挥着至关重要的作用。该转化由整个 OGDHC 催化。OGDC-E 蛋白利用二磷酸硫胺素 (ThDP) 辅助因子促进 2-酮戊二酸（α-酮戊二酸）的不可逆脱羧。然后，它将脱羧酰基中间体转移至与 E2 酶（二氢硫辛酰赖氨酸残基琥珀酰转移酶或 DLST）共价连接的氧化二氢硫辛酰基。这一过程发生在线粒体中，是克雷布斯（柠檬酸）循环中的关键步骤，柠檬酸循环是氧化碳水化合物、脂肪酸和氨基酸等燃料分子的常见途径。虽然 OGDC-E 蛋白可以在体外催化 2-氧代己二酸的脱羧，但与 2-氧代戊二酸相比，其脱羧速度要慢得多。此外，2-酮戊二酸脱氢酶复合物的一部分位于细胞核中，并参与组蛋白的赖氨酸琥珀酰化。它与染色质上的 KAT2A 结合，并向组蛋白琥珀酰转移酶 KAT2A 提供琥珀酰辅酶 A。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-SUMO;N-6*His

Q60597 (695V-1023S)

18293  [NCBI]

VDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLQEENFDINQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPENGPAAQDPHKVKRLLFCTGKVYYDLTRERKARNMEEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS

Approximately 54 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.