Reelin/RELN 蛋白, Human (P.pastoris, His)

Reelin/RELN proteins regulate neuronal delamination in the cerebral cortex and cerebellum, affecting microtubule function and neuronal migration. Reelin/RELN Protein, Human (P.pastoris, His) is the recombinant human-derived Reelin/RELN protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Reelin/RELN Protein, Human (P.pastoris, His) is 229 a.a., with molecular weight of ~26.9 kDa.

Reelin/RELN 蛋白是一种细胞外基质丝氨酸蛋白酶，在大脑皮层和小脑神经元的分层组织中发挥着关键作用。其调节影响延伸到神经元的微管功能和神经元迁移，从而形成复杂的神经网络结构。此外，Reelin/RELN 影响脊髓中交感神经节前神经元的迁移，它似乎充当神经元迁移的屏障。其酶活性对于调节细胞粘附至关重要，强调其参与基本细胞过程。通过与脂蛋白受体 VLDLR 和 LRP8/APOER2 的胞外结构域结合，Reelin/RELN 诱导 DAB1 酪氨酸磷酸化并调节 TAU 磷酸化，表明神经元调节中存在复杂的信号通路。在结构上，它作为二硫键连接的同二聚体的寡聚物存在，并与 VLDLR 和 LRP8/APOER2 的胞外域结合，强调了它在协调分子相互作用中的多方面作用，这对神经的正常发育和组织至关重要。

Human

P. pastoris

N-6*His

P78509 (A26-T254)

5649  [NCBI]

AAGYYPRFSPFFFLCTHHGELEGDGEQGEVLISLHIAGNPTYYVPGQEYHVTISTSTFFDGLLVTGLYTSTSVQASQSIGGSSAFGFGIMSDHQFGNQFMCSVVASHVSHLPTTNLSFIWIAPPAGTGCVNFMATATHRGQVIFKDALAQQLCEQGAPTDVTVHPHLAEIHSDSIILRDDFDSYHQLQLNPNIWVECNNCETGEQCGAIMHGNAVTFCEPYGPRELITT

Approximately 26.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.