RSPO1/R-spondin-1 蛋白, Human (HEK293, His)

RSPO1/R-spondin-1 is a secreted glycoprotein. RSPO1/R-spondin-1 binds to LGR4/5/6 receptors and ZNRF3/E3 ubiquitin ligases, inhibits Wnt receptor degradation, stabilizes β-catenin and promotes its nuclear translocation, and activates target gene transcription. RSPO1/R-spondin-1, Human promotes intestinal organoid survival, induces pancreatic β-cell proliferation to improve diabetes, enhances osteogenic differentiation of mesenchymal stem cells to treat osteoporosis, and inhibits bone erosion in arthritis models. RSPO1/R-spondin-1 Protein, Human (HEK293, His) is a recombinant RSPO1/R-spondin-1 protein expressed by HEK293 with a C-His tag.

RSPO1/R-spondin-1 属于 R-spondin 家族 (含 RSPO 1-4 共 4 个成员)^[2][4]。 RSPO1/R-spondin-1 是一种分泌型糖蛋白。RSPO1/R-spondin-1 与 LGR4/5/6 受体及 ZNRF3/E3 泛素连接酶结合，抑制 Wnt 受体降解，稳定 β-catenin 并促进其核转位，激活靶基因转录^[2][4][7]。RSPO1/R-spondin-1 在肠道上皮、胰腺腺泡、骨骼间充质、关节成骨细胞等组织中表达^[1][5][6][7]。RSPO1/R-spondin-1, Human 与小鼠、大鼠等种属的 RSPO1 在氨基酸序列和功能 (如激活 Wnt 信号、促进组织再生) 上高度同源^[5][6]。RSPO1/R-spondin-1, Human 由 263 个氨基酸组成，含 N-糖基化修饰 (如 Asn137 位点)，糖链结构包括唾液酸、N-乙酰葡糖胺等^[4]。RSPO1/R-spondin-1, Human 促进肠道类器官存活、诱导胰腺 β 细胞增殖改善糖尿病、增强间充质干细胞成骨分化治疗骨质疏松、抑制关节炎模型中的骨侵蚀^[2][5][6][7]。

RSPO1/R-spondin-1, Human (500 ng/mL) 在小鼠肠类器官单元 (OU) 培养中增加尺寸和存活率，激活 Wnt/β-catenin 通路^[2]。
RSPO1/R-spondin-1, Human (1 μg/mL) 诱导人胰腺类器官 (hPOs) 扩增，维持导管细胞表型^[3]。
RSPO1/R-spondin-1, Human (400 nM; 24 h) 促进 MIN6 小鼠胰岛 β 细胞增殖，呈剂量依赖性^[5]。
RSPO1/R-spondin-1, Human (100 ng/mL) 在小鼠 C2C12 间充质细胞和原代成骨细胞中可增强 Wnt 信号 (β-catenin 核积累)，诱导碱性磷酸酶和骨保护素 (OPG) 表达^[7]。

RSPO1/R-spondin-1, Human (0.8 mg/kg; 腹腔注射；80 天) 在 Streptozotocin (HY-13753) 诱导的糖尿病小鼠中维持血糖正常，改善葡萄糖耐量^[5]。
RSPO1/R-spondin-1, Human (3.3 μg/g; 腹腔注射；每日；7 天) 在衰老模型小鼠 (Terc^-/-、Wrn^-/-Terc^-/-) 中提高骨矿化沉积率，促进成骨细胞分化^[6]。

1.R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED₅₀ is 0.5-3 μg/mL.
2.Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 1-10 ng/mL

• 
  R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED₅₀ is 2.232 μg/ml.corresponding to a specific activity is 4.48×10^2 units/mg.

Human

HEK293

C-6*His

Q2MKA7-1 (S21-A263)

284654  [NCBI]

Full Length of Isoform-1 Mature Protein

SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA

Approximately 40-43.3 kDa, based on SDS-PAGE under reducing conditions.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

1.Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.
3.Lyophilized from a 0.2 μm filtered solution of PBS, 8% trehalose, pH 7.4.

<1 EU/μg determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Qu M, et al. Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration. Cell Res. 2021 Mar;31(3):259-271.  [Content Brief]

  [2]. Levin G, et al. R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and Survival in vitro and Improves Tissue-Engineered Small Intestine Formation in vivo. Front Bioeng Biotechnol. 2020 Jun 5;8:476.  [Content Brief]

  [3]. Dossena M, et al. Standardized GMP-compliant scalable production of human pancreas organoids. Stem Cell Res Ther. 2020 Mar 4;11(1):94.  [Content Brief]

  [4]. Levin G, et al. Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells. BMC Biotechnol. 2020 Jan 20;20(1):5.  [Content Brief]

  [5]. Silvano S, et al. RSPO1, a potent inducer of pancreatic β cell neogenesis. Cell Rep Med. 2025 May 20;6(5):102126.  [Content Brief]

  [6]. Wang H, et al. R-Spondin 1 promotes vibration-induced bone formation in mouse models of osteoporosis. J Mol Med (Berl). 2013 Dec;91(12):1421-9.  [Content Brief]

  [7]. Krönke G, et al. R-spondin 1 protects against inflammatory bone damage during murine arthritis by modulating the Wnt pathway. Arthritis Rheum. 2010 Aug;62(8):2303-12.  [Content Brief]