RSPO1/R-spondin-1 蛋白, Mouse (CHO, His)

RSPO1 activates the canonical Wnt signaling pathway by binding to the LGR4-6 receptor, triggering an increase in target gene expression. It inhibits ZNRF3, regulates the Wnt signaling pathway, and negatively regulates the TGF-β pathway. RSPO1/R-spondin-1 Protein, Mouse (CHO, His) is the recombinant mouse-derived RSPO1/R-spondin-1 protein, expressed by CHO , with C-His labeled tag. The total length of RSPO1/R-spondin-1 Protein, Mouse (CHO, His) is 245 a.a., with molecular weight of ~44 kDa.

RSPO1 或 R-spondin-1 蛋白通过充当 LGR4-6 受体的配体，充当经典 Wnt 信号通路的激活剂。与 LGR4-6 结合后，RSPO1 与磷酸化的 LRP6 和卷曲受体结合，这些受体被细胞外 Wnt 受体激活。这种关联触发了经典的 Wnt 信号通路，导致靶基因的表达增加。RSPO1 还通过抑制 ZNRF3（Wnt 信号传导途径的重要调节因子）在调节经典 Wnt/β-连环蛋白依赖性途径和非经典 Wnt 信号传导中发挥作用。此外，RSPO1 作为卷曲 FZD8 和 LRP6 的配体，可能对 TGF-β 途径产生负调节。它在卵巢决定中发挥重要作用，并通过与 KREM1 相互作用拮抗 DKK1/KREM1 介导的 LRP6 内化来调节 Wnt 信号传导。RSPO1 与 ZNRF3 相互作用，促进 ZNRF3 和 LGR4 之间的间接相互作用，并促进 ZNRF3 的膜清除。它还在由 RNF43、LGR5 和 RSPO1 组成的复合物中被识别。RSPO1 与 FZD8 和 LRP6 的胞外结构域相互作用，但不与它们形成三元复合物。此外，RSPO1 与 WNT1 相互作用，结合肝素，并与 LGR4、LGR5 和 LGR6 相互作用。

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells and the ED₅₀ is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL mouse Wnt3a.

Mouse

CHO

C-His

NP_619624.2 (S21-Q265)

192199  [NCBI]

Approximately 44 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.