1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-A
  6. VEGF165 蛋白, Human (HEK293)

VEGF165 蛋白, Human (HEK293)

目录号: HY-P7110A
COA 产品使用指南

VEGF165 蛋白, Human (HEK293)是血管内皮生长因子A (VEGF-A) 的亚型。 VEGF是血管内皮细胞特异性的肝素结合生长因子,能够诱导血管生成。

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规格 价格 是否有货 数量
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2 μg ¥700 In-stock
10 μg ¥1900 In-stock
50 μg ¥5400 In-stock
100 μg ¥8900 In-stock
> 100 μg   询价  

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  • 生物活性

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  • 参考文献

描述

VEGF165 Protein, Human (HEK293) is a Vascular Endothelial Growth Factor A (VEGF-A) isoform. VEGF is a heparin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis.

研究背景

血管内皮生长因子 (VEGF) 具有多种同工型,由选择性剪接或蛋白水解裂解产生,具有不同的受体结合和基质结合特性。已知这些亚型会产生一系列以分支差异为标志的血管生成模式,这对组织具有功能影响。VEGF-AVEGF 细胞因子家族的关键成员,其他成员包括 VEGF-B、-C、-D 和 PlGF。VEGF-A 介导血管生成,即通过新血管的萌芽来扩张现有的血管床。vegfa 基因被翻译成多种剪接亚型,其中在人类中最引人注目的是 VEGF121、VEGF165 和 VEGF189[1]

生物活性

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 this effect is ≤12.99 ng/mL.

  • Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 this effect is 2.538 ng/mL, corresponding to a specific activity is 3.94×105 units/mg.
种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P15692-4 (A27-R191)

基因 ID
蛋白结构
N-term
VEGF165 (A27-R191)
Accession # P15692-4
C-term
同用名
VEGF-AA; rHuVEGF165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen
氨基酸序列

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

分子量

Approximately 17-25 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS or 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 4.0 or 20 mM PB, 150 mM NaCl or 50 mM PB, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

VEGF165 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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