VEGF165 蛋白, Human (HEK293)

VEGF165 Protein, Human is a Vascular Endothelial Growth Factor A (VEGF-A) isoform. VEGF165 Protein is the growth factor that affects the angiogenesis, vasculogenesis and endothelial cell growth. VEGF165 Protein can binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin, and affects the motor neuron signaling pathway. VEGF165 Protein, Human (HEK293) is a human-derived VEGF165 Protein that can be expressed by HEK293.

血管内皮生长因子 (VEGF) 具有多种同工型，由选择性剪接或蛋白水解裂解产生，具有不同的受体结合和基质结合特性。已知这些亚型会产生一系列以分支差异为标志的血管生成模式，这对组织具有功能影响。VEGF-A 是 VEGF 细胞因子家族的关键成员，其他成员包括 VEGF-B、-C、-D 和 PlGF。VEGF-A 介导血管生成，即通过新血管的萌芽来扩张现有的血管床。vegfa 基因被翻译成多种剪接亚型，其中在人类中最引人注目的是 VEGF121、VEGF165 和 VEGF189^[1]。

VEGF165 Protein, Human (50 ng/mL, 12-48 h) 促进 HUVEC 的增殖、迁移、侵袭和管状结构形成^[2]。

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ this effect is <15 ng/mL.

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  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ this effect is 2.538 ng/mL, corresponding to a specific activity is 3.94×10⁵ units/mg.

Human

HEK293

Tag Free

P15692-4 (A27-R191)

7422  [NCBI]

Partial

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 17-25 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 4.0 or 50 mM PB, 300 mM NaCl, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose or 20 mM Tris, 150 mM NaCl, pH 8, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Vempati P, et al. Extracellular regulation of VEGF: isoforms, proteolysis, and vascular patterning. Cytokine Growth Factor Rev. 2014 Feb;25(1):1-19.  [Content Brief]

  [2]. Dan H, et al., CM082, a novel VEGF receptor tyrosine kinase inhibitor, can inhibit angiogenesis in vitro and in vivo. Microvasc Res. 2021 Jul;136:104146.  [Content Brief]