1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. VEGF & VEGFR Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. VEGFR
  5. VEGFR-3
  6. VEGFR-3/FLT4 蛋白, Human (HEK293, His)

VEGFR-3/FLT4 蛋白, Human (HEK293, His)

目录号: HY-P74468
COA 产品使用指南

VEGFR-2蛋白是一种重要的酪氨酸蛋白激酶受体,作为VEGFA、VEGFC和VEGFD的细胞表面受体,调节血管生成、血管发育和胚胎造血。它增强内皮细胞增殖、存活、迁移和分化。VEGFR-3/FLT4 蛋白, Human (HEK293, His) 是重组的 VEGFR-3/FLT4 蛋白,由 HEK293 表达,带有 C-His 标签。VEGFR-3/FLT4 蛋白, Human (HEK293, His) 全长 752 个氨基酸,分子量约为 ~130 kDa。

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5 μg ¥780 In-stock
10 μg ¥1250 In-stock
20 μg ¥2000 In-stock
50 μg ¥3800 In-stock
100 μg   询价  

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描述

VEGFR-2 protein is an important tyrosine protein kinase receptor that serves as a cell surface receptor for VEGFA, VEGFC and VEGFD to regulate angiogenesis, blood vessel development and embryonic hematopoiesis. It enhances endothelial cell proliferation, survival, migration and differentiation. VEGFR-3/FLT4 Protein, Human (HEK293, His) is the recombinant human-derived VEGFR-3/FLT4 protein, expressed by HEK293 , with C-His labeled tag. The total length of VEGFR-3/FLT4 Protein, Human (HEK293, His) is 752 a.a., with molecular weight of ~130 kDa.

研究背景

VEGFR-2蛋白是一种酪氨酸蛋白激酶受体,在调节血管生成、血管发育、血管通透性和胚胎造血等多种过程中发挥着至关重要的作用。它作为 VEGFA、VEGFC 和 VEGFD 的细胞表面受体,促进内皮细胞的增殖、存活、迁移和分化。此外,缺乏跨膜结构域的异构体(例如异构体 2)充当 VEGFA、VEGFC 和/或 VEGFD 的诱饵受体,通过抑制这些生长因子与 FLT4 的结合,充当淋巴管生成的负调节因子。VEGFR-2 还与 FLT1 和 FLT4 形成异二聚体,调节它们的信号传导。血管生长因子激活异构体 1 会触发多个信号级联反应,包括激活 PLCG1,从而产生二酰基甘油和肌醇 1,4,5-三磷酸,以及激活蛋白激酶 C。它还会激活 MAPK1/ ERK2、MAPK3/ERK1 和 AKT1 信号通路,导致各种细胞反应。此外,VEGFR-2磷酸化PIK3R1,促进磷脂酰肌醇3-激酶的激活,并促进肌动蛋白细胞骨架重组和PTK2/FAK1的激活。它在 NOS2 和 NOS3 诱导中的作用导致内皮细胞产生一氧化氮 (NO)。此外,VEGFR-2 磷酸化 PLCG1 并促进 FYN、NCK1、NOS3、PIK3R1、PTK2/FAK1 和 SRC 磷酸化,进一步促进其多样化的细胞功能。

生物活性

1.Measured by its binding ability in a functional ELISA.
2. Immobilized human VEGF-C at 10 μg/mL (100 μl/wel) can bind human VEGFR3-his The EC50 of human VEGFR3-his is 0.011 μg/mL.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P35916 (Y25-I776)

基因 ID
蛋白结构
N-term
VEGFR-3 (Y25-I776)
Accession # P35916
His
C-term
同用名
Vascular endothelial growth factor receptor 3; VEGFR-3; FLT-4
分子量

Approximately 130 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as sterile PBS. pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VEGFR-3/FLT4 Protein, Human (HEK293, His)
目录号:
HY-P74468
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