2. Epigenetics
  3. TET Protein
  4. Recombinant Ten-Eleven Translocase

Recombinant Ten-Eleven Translocase 是一种重组表达的 Fe(II) 和 α-酮戊二酸依赖性的双加氧酶。Recombinant Ten-Eleven Translocase 通过一系列氧化反应，可将 5-甲基胞嘛啶 (5mC) 转化为 5-羟甲基胞嘱啶 (5hmC)，再转化为 5-甲酰胞嘧啶 (5fC) 和 5-羧基胞嘘啶 (5caC)。然后再通过转化剂或酶法的作用，5caC 进一步被转化成尿嘧啶 (U)，通过 PCR 将尿嘧啶 (U) 转化成胸腺嘧啶 (T)，从而达到单碱基分辨率和高准确性的 DNA 甲基化位点鉴定。

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Recombinant Ten-Eleven Translocase

Recombinant Ten-Eleven Translocase Chemical Structure

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货
1 mg ￥2360
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Recombinant Ten-Eleven Translocase 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Recombinant Ten-Eleven Translocase is a recombinant Fe(II)- and α-ketoglutarate-dependent dioxygenase. Recombinant Ten-Eleven Translocase converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and then to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) through a series of oxidation reactions. Then, 5caC is further converted into uracil (U) through the action of a conversion agent or enzyme, and uracil (U) is converted into thymine (T) through PCR, thereby achieving single-base resolution and high-accuracy identification of DNA methylation sites[1].

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Recombinant Ten-Eleven Translocase 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Recombinant Ten-Eleven TranslocaseTET ProteinTET enzymeTen-eleven-translocation (TET) proteinTen-eleven translocation (TET) methylcytosine dioxygenasedioxygenaseDNA methylation sitesInhibitorinhibitorinhibit

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