2. Immunology/Inflammation
  3. SphK
  4. SK1-I

SK1-I  (Synonyms: BML-258)

目录号: HY-119016 纯度: 98.47%
COA 产品使用指南

摩尔计算器

SK1-I (BML-258) 是鞘氨醇的类似物，是同功酶特异性 SPHK1 竞争抑制剂，Ki 值为 10 µM。SK1-I 对 SPHK2，PKCα，PKCδ，PKA，AKT1，ERK1，EGFR，CDK2，IKKβ 或 CamK2β 无活性。SK1-I 增强自噬并具有抗肿瘤活性。

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SK1-I Chemical Structure

SK1-I Chemical Structure

CAS No. : 1072443-89-0

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规格 价格 是否有货 数量
1 mg ￥1590
In-stock
5 mg ￥3500
In-stock
10 mg ￥5800
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of SK1-I:

SK1-I 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

SK1-I (BML-258), an analog of sphingosine, is an isozyme-specific competitive SPHK1 inhibitor with a Ki value of 10 µM[1]. SK1-I shows no activity at SPHK1 PKCα, PKCδ, PKA, AKT1, ERK1, EGFR, CDK2, IKKβ or CamK2β. SK1-I enhances autophagy and has antitumor activity[2].

IC50 & Target

Ki: 10 µM (SPHK1)[1]
体外研究
(In Vitro)

SK1-I (0-10 μM; 24 hours) attenuates cancer cell growth and survival in a TP53-dependent manner in HCT116 cells and HCT116 cells bearing TP53 null cancer[2].
SK1-I (0-20 μM; 12 hours) induces more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53, leading to a hallmark of apoptosis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

SK1-I 相关抗体:

Cell Viability Assay[2]

Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer
Concentration: 0 µM, 2.5 µM, 5 µM, 7.5 µM, 10 µM
Incubation Time: 24 hours
Result: Decreased cancer cell growth and survival.

Western Blot Analysis[2]

Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer
Concentration: 0 µM, 5 µM, 10 µM, 20 µM
Incubation Time: 12 hours
Result: Induced more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53.
体内研究
(In Vivo)

Pre-treatment with SK1-I (BML-258; intraperitoneal (i.p.) injection; once; 24 hours prior to baseline mean arterial blood pressure (MAP) measurement; 75 mg/kg) before anandamide (i.v. injection; two doses; 1 and 10 mg/kg) significantly decreases the hypotensive response[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (24 ± 3.5 g) [3]
Dosage: 75 mg/kg
Administration: Intraperitoneal (i.p.) injection; once; 24 hours prior to baseline MAP measurement
Result: Significantly lowered baseline mean arterial blood pressure (MAP).
分子量

277.40

Formula

C17H27NO2
CAS 号
性状

固体

颜色

Off-white to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (360.49 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.6049 mL 18.0245 mL 36.0490 mL
5 mM 0.7210 mL 3.6049 mL 7.2098 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

SK1-I 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6049 mL 18.0245 mL 36.0490 mL 90.1226 mL
5 mM 0.7210 mL 3.6049 mL 7.2098 mL 18.0245 mL
10 mM 0.3605 mL 1.8025 mL 3.6049 mL 9.0123 mL
15 mM 0.2403 mL 1.2016 mL 2.4033 mL 6.0082 mL
20 mM 0.1802 mL 0.9012 mL 1.8025 mL 4.5061 mL
25 mM 0.1442 mL 0.7210 mL 1.4420 mL 3.6049 mL
30 mM 0.1202 mL 0.6008 mL 1.2016 mL 3.0041 mL
40 mM 0.0901 mL 0.4506 mL 0.9012 mL 2.2531 mL
50 mM 0.0721 mL 0.3605 mL 0.7210 mL 1.8025 mL
60 mM 0.0601 mL 0.3004 mL 0.6008 mL 1.5020 mL
80 mM 0.0451 mL 0.2253 mL 0.4506 mL 1.1265 mL
100 mM 0.0360 mL 0.1802 mL 0.3605 mL 0.9012 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SK1-I1072443-89-0BML-258BML258BML 258SphKSphingosine kinasecompetitiveantitumorautophagyInhibitorinhibitorinhibit

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目录号：
HY-119016
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