Thermolysin, Bacillus thermoproteolyticus rokko  (Synonyms: EC 3.4.24.27; TML)

Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues^[1].

Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
Optimal temperature : 70 °C

Thermolysin (1 mL 中 100 μg，64°C，4 小时，在分离的淀粉颗粒中) 消化可选择性去除一组 10 至 27 kD 之间的低分子量蛋白质^[2].
Thermolysin 可用于水解牛肝肌浆蛋白^[3]。
产品信息
分子量：34.6 kDa (calculated)
最佳 pH：8 0，在 pH 5.0-9 5 范围内稳定
最佳温度：70℃
等电点：4.45

使用说明
可溶于醋酸盐缓冲液，pH 值为 7.5（0.2 mg/mL），形成澄清至略带浑浊的无色溶液。或根据具体实验参考文献使用纯水或 buffer 溶解。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Thermolysin（50 mg/kg，口服，一次）可降低 SHR 大鼠的收缩压^[4]。
Thermolysin 不具有急性毒性，大鼠经口 LD₅₀ 超过 18000 mg/kg，小鼠经口 LD₅₀ 超过 24000 mg/kg（急性毒性研究）^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9073-78-3

固体

White to light yellow

嗜热菌蛋白酶

3.4.24.27

30-350 U/mg protein

One unit is defined as the amount of hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) 7.5g of tyrosine per min at pH 7.5 at 37°C.(Folin Jockett reagent)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bertusvan den Burg, et al. Chapter 111 - Thermolysin and Related Bacillus Metallopeptidases.

  [2]. Mu-Forster C, et al. Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides. Plant Physiol. 1998 Apr;116(4):1563-71.  [Content Brief]

  [3]. Di Bernardini R, et al. Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides. 2011 Feb;32(2):388-400.  [Content Brief]

  [4]. Fujita H, et al: a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology. 1999 Oct 15;44(1-2):123-7.  [Content Brief]

  [5]. Ke Q, et al. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus. Food Chem Toxicol. 2013 Sep;59:541-8.  [Content Brief]