2. Others
  3. Fluorescent Dye
  4. YO-PRO-1

YO-PRO-1 是一种非细胞膜穿透性的绿色荧光染料。YO-PRO-1 不能渗透正常的细胞膜，但可以渗透凋亡和坏死细胞的细胞膜。YO-PRO-1 对 DNA 具有高亲和力，在未与 DNA 结合时基本上不发出荧光，与 DNA 结合后可发出绿色荧光 (Ex/Em: 488/530±30 nM)。YO-PRO-1 常与 Propidium Iodide (HY-D0815) 一起用来分析和鉴定凋亡和坏死细胞。

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YO-PRO-1

YO-PRO-1 Chemical Structure

CAS No. : 152068-09-2

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规格 价格 是否有货 数量
100 μg ￥2486
In-stock
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1 mg 现货 询价
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YO-PRO-1 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

YO-PRO-1 is a non-cell-membrane-penetrating green fluorescent dye. YO-PRO-1 cannot penetrate normal cell membranes but can penetrate the membranes of apoptotic and necrotic cells. YO-PRO-1 has a high affinity for DNA. When it is not bound to DNA, it emits almost no fluorescence, and when it binds to DNA, it can emit green fluorescence (Ex/Em: 488/530±30 nM). YO-PRO-1 is often used together with Propidium Iodide (HY-D0815) to analyze and identify apoptotic and necrotic cells[1][2].

体外研究
(In Vitro)

YO-PRO-1 染色的简单步骤: 准备细胞/组织样本; YO-PRO-1 孵育 30 min-1 h 后洗涤，可与 Hoechst 33342 (HY-15559)、Propidium Iodide (HY-D0815) 等共染; 显微镜观察和检测: 用 488 nm 激光线激发成像[1][2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

YO-PRO-1 相关抗体:
分子量

629.32

Formula

C24H29I2N3O
CAS 号
性状

固体

颜色

Yellow to orange

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

纯度 & 产品资料

纯度: 98.20%

COA (288 KB)

产品使用指南 (1538 KB)
参考文献

YO-PRO-1 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

YO-PRO-1152068-09-2Fluorescent DyeFluorochromeDNAapoptotic cellsnecrotic cellsInhibitorinhibitorinhibit

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目录号：
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