1. Membrane Transporter/Ion Channel Autophagy Anti-infection Apoptosis
  2. Proton Pump Autophagy Antibiotic Bacterial Apoptosis
  3. Bafilomycin A1

Bafilomycin A1  (Synonyms: 巴佛洛霉素A1; BafA1)

目录号: HY-100558 纯度: 98.47%
COA 产品使用指南

Bafilomycin A1 (BafA1) 是特异性,可逆的 V-ATPase 抑制剂,IC50 值为 4-400 nmol/mg。Bafilomycin A1,大环内酯类抗生素, 是一种自噬 (autophagy) 晚期阶段抑制剂。Bafilomycin A1 阻断自噬体与溶酶体的融合,并抑制培养细胞溶酶体中的酸化和蛋白质降解。Bafilomycin A1 也诱导调亡 (apoptosis)。

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Bafilomycin A1 Chemical Structure

Bafilomycin A1 Chemical Structure

CAS No. : 88899-55-2

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Customer Review

MCE 顾客使用本产品发表的 381 篇科研文献

WB
IF
Proliferation Assay

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nature. 2022 Aug;608(7922):413-420.  [Abstract]

    Huh7 cells and ASGR1 KO cells are treated with 20 nM Bafilomycin A1 for 2 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844.  [Abstract]

    Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30 nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844.  [Abstract]

    Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30 nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Nanotechnol. 2022 Sep;17(9):993-1003.  [Abstract]

    Immunofluorescence (IF) images of phagocytosis and elimination of SC2-P (red) in the absence or presence of CIPS in THP-1 differentiated macrophages. The lysosomal inhibitor Bafilomycin A1 (BM; 100 nM; 6 h) is added to macrophages 6 h before the end of culture.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    The levels of mitochondrial proteins including TIM23, TOM20, and TOM22 decreased along with decreased O-GlcNAcylation levels, which can be inhibited by BafA1.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Mil Med Res. 2022 Feb 14;9(1):9.  [Abstract]

    GL261 and U251 cells are treated with 3-MA (5 mM), BafA1 (10 nM), AG-205 (10 µM), or RAPA (20 nM) for 1 h followed by IR or IR plus UTMD treatment for another 24 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Biomaterials. 2022 Sep;288:121743.

    In response to the Bafilomycin A1 (Baf; 50 nM) treatment, increases LC3 II expression is observed in PDLSCs, and significant differences between the Baf-treated PDLSCs and cells without the Baf treatment are observed at 6 h and 12 h during osteogenic induction.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Biomaterials. 2022 Sep;288:121743.

    Bafilomycin A1 (Baf; 50 nM) treatment significantly increases the percentage of both RFP+-GFP+-LC3 puncta and LC3 puncta in PDLSCs at 12 and 24 h during osteogenic induction, while there is no significant difference in the percentage of RFP+-GFP+-LC3 puncta or LC3 puncta between the Baf-treated I-PDLSCs and cells without the Baf treatment.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Feb 17;6(1):67.  [Abstract]

    Representative images of cells with GFP or GFP-LC3 puncta, and the average number of GFP-LC3 puncta per cell in stable RFP-GFP-LC3 U87 cells treated with Justicidin A (JA) (0.13 μM), Justicidin B (JB) (0.13 μM), or Justicidin C (JC) (4 μM) in the presence or absence of Compound C (2.5 μM), SCH772984 (10 μM), LY294002 (25 μM), or Bafilomycin A1 (1 nM) for 24 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Metab. 2021 May 4;33(5):971-987.e6.  [Abstract]

    Western blot detection of YAP expression in HEK293T cells transfected with siControl or siATP6V0d2, followed by treatment with 100 nM Bafilomycin A1 (BafA1) for 6 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Mol Immunol. 2022 Jan;19(1):67-78.  [Abstract]

    HeLa cells transfected with the ORF10-GFP plasmid and pEGFP-N1 are stimulated by poly(I:C) transfection and are then treated with MG132 (5 μM), Bafilomycin A1 (Baf A1; 5 nM), or Chloroquine (CQ; 40 μM) for 24 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Stem Cell. 2021 Jun 3;28(6):1074-1089.e7.  [Abstract]

    Viability determined by cell counts of P, SA, and SAR iPSC-HSPCs treated with the V-ATPase inhibitor Bafilomycin A1 (Baf A1; 10 nM; 48 hours) compared with untreated cells.

    Bafilomycin A1 purchased from MCE. Usage Cited in: J Extracell Vesicles. 2021 Oct;10(12):e12153.  [Abstract]

    Confocal microscopy analysis of the MVB marker CD63 in Coro1a‐Flag or Coro1a‐K233R‐Flag overexpressing HeLa cells treated with DMSO or 20 nM Bafilomycin A1 (Baf A1) for 12 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 3;9(10):1015.  [Abstract]

    Representative western blot images of LC3 (LC3I and LC3II) in primary PTC are isolated from WT and TRPC6-/- mice after treatment with H2O2 (0.5 mM 12 h) in the presence and absence of Bafilomycin A1 (BAF) (20 nM).

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195.  [Abstract]

    Western analysis of the effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without Bafilomycin A1 (Baf, 10 nM) in HEK293T cells.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195.  [Abstract]

    Western analysis of the effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without Bafilomycin A1 (Baf, 10 nM) in osteoclasts.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing SQSTM1 and LC3 levels in LN229 cells after treatment with 200 μM NSC 697855 (NTZ) and 200 nM Bafilomycin A1 (BAF) for 48 h.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Bafilomycin A1 (BafA1) is a specific and reversible inhibitor of vacuolar H+-ATPase (V-ATPase) with IC50 values of 4-400 nmol/mg. Bafilomycin A1, a macrolide antibiotic, is also used as an autophagy inhibitor at the late stage. Bafilomycin A1 blocks autophagosome-lysosome fusion and inhibits acidification and protein degradation in lysosomes of cultured cells. Bafilomycin A1 induces apoptosis[1][2][3].

    IC50 & Target

    Macrolide

     

    体外研究
    (In Vitro)

    Bafilomycin A1 is treated to different types of membrane ATPases with the I50 of 400 nmol/mg, 4 nmol/mg and 50 nmol/mg for the vacuolar ATPases of a fungus (N. crassa), a plant (Z. mays), and an animal (bovine abrenal medulla). The I50 values refer as μmol of Bafilomycin A1 per mg of protein giving 50% inhibition of ATPase activity[1].
    Bafilomycin A1 ((-)-Bafilomycin A1) disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion[2].
    Bafilomycin A1 at a low concentration (1 nM) effectively and specifically inhibits and kills pediatric B-cell acute lymphoblastic leukemia cells. It targets both early and late stages of the autophagy pathway, mitochondria and induces caspase-independent apoptosis. Bafilomycin A1 induces the binding of Beclin 1 to Bcl-2, which further inhibits autophagy and promotes apoptotic cell death[5].
    The growth of the BEL-7402 hepatocellular carcinoma and HO-8910 ovarian cancer cell lines are retarded and the metastatic potential is inhibited by Bafilomycin A1. Transmission electron microscopy and assays of capsase-3 and -9 suggest that Bafilomycin A1 induces apoptosis[6].
    Bafilomycin A1 inhibits the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they are transformed, and PC12 and HeLa cells. The IC50 of Bafilomycin A1 for inhibition of cell growth ranges from 10 to 50 nM[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Chronic treatment with low-dose Bafilomycin A1 (0.1 mg/kg) slightly inhibits the tumor volume, but the final tumor volume does not differ significantly from the control. However, chronic treatment with high dose Bafilomycin A1 (1 mg/kg) inhibits the tumor growth significantly, compared with controls, after 21 days[8].
    Bafilomycin A1 (0.1 mg/kg or 1 mg/kg; i.p. daily for 3 days) extends the survival of B-cell acute lymphoblastic leukemia (B-ALL) xenograft mice with advanced disease[9].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    622.83

    Formula

    C35H58O9

    CAS 号
    性状

    固体

    颜色

    White to light yellow

    中文名称

    巴佛洛霉素A1;巴弗洛霉素A1

    结构分类
    初始来源

    Streptomyces griseus strains

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    细胞实验: 

    DMSO 中的溶解度 : 50 mg/mL (80.28 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.6056 mL 8.0279 mL 16.0557 mL
    5 mM 0.3211 mL 1.6056 mL 3.2111 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (4.01 mM); 悬浊液; 超声助溶

      此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.34 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.43%

    参考文献
    Cell Assay
    [2]

    Cells are harvested using 0.05% trypsin and suspended in culture medium containing 10% FCS, and 200 µL suspension is added to each well of a 96-well plate. Cells are cultured for 20 h for adhesion. Bafilomycin A1 is added to the wells at the final concentrations of 200, 400 and 800 nM, in triplicate. At 24, 48 and 72 h, 20 µl WST-1 is added to the cells. Following incubation at 37°C for 4 h, the plates are read to determine the optical density (OD) at 435 nm with 675 nm reference using a spectrophotometer[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4]

    Mice: Tumor-bearing mice are divided randomly into three experimental groups: a low-dose Bafilomycin A1 (0.1 mg/kg per day)-treated group (n=5), a high-dose Bafilomycin A1 (1 mg/kg per day)-treated group (n=5),and a control group (n=5). Tumor size is measured and tumor volume doubling time is calculated[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6056 mL 8.0279 mL 16.0557 mL 40.1394 mL
    5 mM 0.3211 mL 1.6056 mL 3.2111 mL 8.0279 mL
    10 mM 0.1606 mL 0.8028 mL 1.6056 mL 4.0139 mL
    15 mM 0.1070 mL 0.5352 mL 1.0704 mL 2.6760 mL
    20 mM 0.0803 mL 0.4014 mL 0.8028 mL 2.0070 mL
    25 mM 0.0642 mL 0.3211 mL 0.6422 mL 1.6056 mL
    30 mM 0.0535 mL 0.2676 mL 0.5352 mL 1.3380 mL
    40 mM 0.0401 mL 0.2007 mL 0.4014 mL 1.0035 mL
    50 mM 0.0321 mL 0.1606 mL 0.3211 mL 0.8028 mL
    60 mM 0.0268 mL 0.1338 mL 0.2676 mL 0.6690 mL
    80 mM 0.0201 mL 0.1003 mL 0.2007 mL 0.5017 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Bafilomycin A1
    目录号:
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