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Safflower yellow  (Synonyms: 红花黄)

目录号: HY-N0938
COA 产品使用指南

Safflower yellow 是从植物红花 (Carthamus tinctorius) 的花中提取的,已被用于心脑血管疾病的相关研究。

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Safflower yellow Chemical Structure

Safflower yellow Chemical Structure

CAS No. : 1401-20-3

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Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

WB

    Safflower yellow purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Nov;107:1736-1743.  [Abstract]

    Western blot is used to assess the protein levels of VEGF, Ang-2, ALP, Runx2, and OPN-1 in the co-culture of HUVEC-12 and BMSCs. β-actin is used as a loading control.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.

    体内研究
    (In Vivo)

    Safflower yellow (SY) is the safflower extract and is the one of traditional Chinese medicine. Safflower yellow can promote blood circulation, remove blood stasis, and thereby improve capillary circulation at the site of tissue injury. Safflower yellow is mixtures of a water-soluble chalcone component, in which both hydroxyl safflower yellow A (HSYA) and safflower yellow B (SYB) are the main components. Safflower injection excellently protects the heart by way of improving functions of cardiac contraction and dilation, increasing coronary blood flow, and strengthening the bcl-2 (anti apoptosis gene) protein expression. Safflower yellow alleviates the injured tendon adhesion and inflammatory reaction and promoted the repair of injured tendon[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    CAS 号
    性状

    固体

    颜色

    Light brown to brown

    Emission (Em)

    500

    Excitation (Ex)

    320

    中文名称

    红花黄

    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 20 mg/mL (超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2 mg/mL (Infinity mM); 澄清溶液

      此方案可获得 ≥ 2 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2 mg/mL (Infinity mM); 澄清溶液

      此方案可获得 ≥ 2 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料
    参考文献
    Animal Administration
    [1]

    Rabbits[1]
    The adult male New Zealand rabbits (body weight 2.0-2.5 kg) are used. Twenty-four rabbits are randomly divided into three groups (per group): sham-operated control (Cont), spinal cord ischemia reperfusion, and treated with safflower yellow. The control group only execute anesthesia and surgical procedures, except for occluding the abdominal aorta. The group is intravenously injected with 2 mL/kg of a solution of 16% (wt/vol) Safflower yellow (1 mL, containing 1.6 mg Safflower yellow), followed by continuous infusion of a total of 5 mL/kg through the right femoral vein at the moment of reperfusion beginning after 40 minutes of the abdominal aorta occlusion. The same volumes of 0.9% saline solution are administrated in control and groups. Blood samples are obtained at the end of 0 hour, 4 hours, 12 hours, 24 hours, and 48 hours after reperfusion, and the plasma is separated and stored at -80°C for further analysis. All animals are sacrificed 48 hours after reperfusion and are rapidly perfused with 0.9% sodium chloride, and the L2-5 segments of the spinal cord are quickly removed. The L2-3 segment in each animal is used for western blot, and the other segment (L4-5) is immersed into 10% neutral formaldehyde for 2-3 days and is used for morphology analysis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    Safflower yellow 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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