AkaLumine 

AkaLumine is a D-luciferin (HY-12591A) analogue with a K_m of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine has high tissue-penetration and increases detection sensitivity from deep-tissue targets^[1].

Km: 2.06 μM^[1].

1. 溶液配置^[2]
1.1 制备储存液
溶剂：无水 DMSO 或超纯水。
浓度：建议 10 mM (如：称取 3.17 mg AkaLumine hydrochloride，溶于 1 mL 溶剂)。
保存：分装后 -20°C 避光保存，避免反复冻融。
1.2 工作液的配制
用 PBS 或无血清细胞培养基稀释至 1-100 μM (根据实验优化)。
注意：工作液现配现用，避光。

2. 细胞染色
2.1 将细胞用 100 μL PBS 分散并悬浮于 96 孔板中，密度为 2×10⁵。
2.2 将 AkaLumine hydrochloride (100 µM) 和 5 mM ATP magnesium (HY-B2176) 加入细胞中。
2.3 使用生物发光成像系统进行分析。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

皮下/原位肿瘤小鼠成像^[1]
1. 将肿瘤细胞经皮下或胫骨内注射到小鼠体内。
2. 腹腔注射不同浓度的 AkaLumine hydrochloride (0.5-33 mM; 100 μL)。
3. 注射 15 分钟后用生物发光仪检测。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

302.39

C₁₆H₁₈N₂O₂S

1176235-08-7

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856.  [Content Brief]

  [2]. Zhou M, et al. Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology. Stem Cell Res. 2021 Oct;56:102532.  [Content Brief]