ATG10 抗体

ATG10 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to ATG10.

ATG10：自噬是溶酶体大量降解胞质隔室的过程。ATG10 是一种 E2 样酶，参与自噬体形成所必需的 2 种泛素样修饰：ATG12 (MIM 609608)-ATG5 (MIM 604261) 可溶性形式 MAP-LC3 (MAP1LC3A；MIM 601242) 的缀合和修饰，一种同系物酵母 Apg8 转化为膜结合形式（Nemoto 等人，2003 [PubMed 12890687]）。[由 OMIM 提供，2008 年 3 月]

Q9H0Y0

Predicted band size: 25 kDa; Observed band size: 25 kDa

Affinity Purified

Cytoplasm.

Non-conjugated

Unmodified

AB_3103115

Cell Biology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20ug) ,HEK293T(lane 3(20ug) and K562(lane 4(20ug) using ATG10 Antibody (HY-P81236) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P

液体

Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Fusion protein of human ATG10