1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. Band 3 抗体 (YA1819)

Band 3 抗体 (YA1819)

目录号: HY-P82074
COA 抗体使用指南 技术支持

Band 3 Antibody (YA1819) 是一个兔来源、无偶联标记、抗 Band 3 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Band 3 Antibody (YA1819) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Band 3.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 102 kDa;
Observed band size: 102 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human Band 3 / CD233 aa20-70/911.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
敏感性 Endogenous 纯度 Affinity Chromatography
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of Hela cells labeling Band 3 with Band 3 Antibody (HY-P82074) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Band 3 Antibody (HY-P82074) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling Band 3 with Band 3 Antibody (HY-P82074) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Band 3 Antibody (HY-P82074) at 1/100 at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID
反应种属数据库
研究领域

Cardiovascular

中文名
Band 3 抗体 (YA1819)
同用名
AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SLC4A1; SW; WD; WD1; WR
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Band 3 Antibody (YA1819)
目录号:
HY-P82074
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