Cyclophilin A 抗体 (YA481)

Cyclophilin A Antibody (YA481) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclophilin A.

亲环蛋白 A 催化脯氨酸酰亚胺肽键的顺反异构化，通过激活其膜受体 BSG/CD147 对白细胞发挥有效的趋化作用，并启动信号级联反应，最终导致 MAPK/ERK 激活。该蛋白通过刺激 NF-kappa-B 和 MAP 激酶信号传导，以促炎方式激活内皮细胞 (EC)，诱导 SELE 和 VCAM1 等粘附分子的表达。此外，Cyclophilin A 通过促进 CCL2 和 BCL2L11 的 FOXO1 依赖性表达来诱导 EC 细胞凋亡。为了应对氧化应激，它通过 NF-kappa-B 激活、AKT1 上调和 BCL2 诱导启动 EC 中的促凋亡和抗凋亡信号通路。它负向调节 MAP3K5/ASK1 激酶活性，对于 TARDBP 在异质核核糖核蛋白复合物中的组装至关重要，从而影响 TARDBP 与 RNA 的结合以及 HDAC6、ATG7 和 VCP 的表达，这对于蛋白质聚集物的清除至关重要。亲环蛋白 A 在血小板活化和聚集、调节钙动员、整合素 ITGA2B:ITGB3 双向信号传导以及结合硫酸乙酰肝素糖胺聚糖方面也发挥着关键作用。

Free

P62937

Predicted band size: 18 kDa；Observed band size: 15 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Secreted.

Non-conjugated

Unmodified

AB_3102823

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Cyclophilin A Antibody (HY-P80101). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Cyclophilin A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80101, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Cyclophilin A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80101, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Cyclophilin A.AA range:131-159.