EAAT3 抗体 (YA2365)

EAAT3 Antibody (YA2365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EAAT3.

EAAT3 是一种钠依赖性高亲和力氨基酸转运蛋白，主要介导 L-glutamate、L-aspartate 和 D-aspartate 的摄取，同时也能转运 L-cysteine。它作为同向转运体（symporter），在转运一个氨基酸分子的同时伴随 2-3 个 Na(+) 离子和 1 个质子的同向运输，并反向转运 1 个 K(+) 离子。该蛋白还能介导与氨基酸转运无关的 Cl(-) 流动，以避免天冬氨酸和 Na(+) 同向转运导致的负电荷积累。在肾小管中对 L-glutamate 和 L-aspartate 的重吸收起重要作用。类似功能（by similar）包括：在突触间隙快速清除释放的谷氨酸以终止突触后谷氨酸作用中的冗余角色，以及通过转运 L-glutamate 和 L-cysteine 参与谷胱甘肽生物合成和抗氧化应激。其活性受 ARL6IP5 负调控。

P43005

Predicted band size: 57 kDa; Observed band size: 70 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104720

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Immunocytochemistry analysis of SH-SY5Y cells labeling EAAT3 Antibody (HY-P82620) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EAAT3 Antibody (HY-P82620) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of SH-SY5Y cells labeling EAAT3 Antibody (HY-P82620) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EAAT3 Antibody (HY-P82620) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using EAAT3 Antibody (YA2365). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82620, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using EAAT3 Antibody (YA2365). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82620, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human EAAT3