1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. GCLC 抗体 (YA2898)

GCLC 抗体 (YA2898)

目录号: HY-P83153
COA 抗体使用指南 技术支持

GCLC Antibody (YA2898) 是一个兔来源、无偶联标记、抗 GCLC 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

GCLC Antibody (YA2898) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCLC.

研究背景

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The locus encodes the catalytic subunit, while the regulatory subunit is derived from a different related gene located on chromosome 1p22-p21. Mutations at The locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

基因 ID
蛋白数据库
中文名
GCLC 抗体 (YA2898)
同用名
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
分子量

Predicted band size: 73 kDa; Observed band size: 73 kDa

纯度

Affinity Purified

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Signal Transduction

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:1000

  • Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
应用

WB

性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

Recombinant protein of human GCLC

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
GCLC Antibody (YA2898)
目录号:
HY-P83153
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