Ku70 抗体 (YA715)

Ku70 Antibody (YA715) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Ku70.

Ku70 是一种单链 DNA 依赖性 ATP 依赖性解旋酶，在 DNA 非同源末端连接 (NHEJ) 中起关键作用，通过将 DNA-PK 招募到 DNA 上参与双链断裂修复和 V(D)J 重组。它还参与染色体易位，并以细胞周期依赖性方式优先结合双链 DNA 的叉状末端，沿 3'-5' 方向工作。在 NHEJ 过程中，XRCC5-XRRC6 二聚体识别并结合 DNA 的断裂末端，保护其免受进一步切除，并通过将 PRKDC 对 DNA 的亲和力提高 100 倍来调节 DNA-PK 复合物的活性。此外，Ku70 可能还具有 5'-脱氧核糖-5-磷酸裂解酶 (5'-dRP lyase) 活性，可清除脱碱基位点的末端。它与 APEX1 共同作为转录的负调节因子，并与 NAA15 结合激活骨钙素表达。Ku70 还通过组装成 HDP-RNP 复合物调节 DNA 病毒介导的先天免疫反应，并通过与 BAX 相互作用抑制凋亡。

Free

P12956

Predicted band size: 70 kDa;Observed band size: 75 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102855

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:2000; IHC-P: 1:100-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), A549 (lane 3(20μg) and HEK293(lane 4(20μg) using Ku70 (HY-P80202) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Ku70 Antibody (YA715). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80202, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Ku70 Antibody (YA715). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80202, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human Ku70.AA range:1-150.