Mast Cell Tryptase 抗体 (YA1533)

Mast Cell Tryptase Antibody (YA1533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mast Cell Tryptase.

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase related genes are clustered on chromosome 16p13.3. These related genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These related genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. The feature is characteristic of tryptases but is unusual in other related genes. The alleles of The related gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate related genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathorelated genesis of asthma and other allergic and inflammatory disorders.

Q15661

Predicted band size: 31 kDa; Observed band size: 31 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:50

• 
  Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), and Mouse liver (lane 2, 15 μg) using Tryptase I Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Mast Cell Tryptase