MBD2 抗体 (YA2279)

MBD2 Antibody (YA2279) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBD2.

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated related gene promoters. The protein encoded by The related gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the The protein functions as a demethylase to activate transcription, as DNA methylation causes related gene silencing. Two transcript variants encoding different isoforms have been found for The related gene.

Q9UBB5

Predicted band size: 43 kDa; Observed band size: 43,32,29 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), A431 (lane 2, 15 μg), A375 (lane 3, 15 μg), and 3T3 (lane 4, 15 μg) using MBD2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human MBD2