Phospho-AMPK alpha 1 (Ser496) 抗体

Phospho-AMPK alpha 1 (Ser496) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-AMPK alpha 1 (Ser496).

AMPK alpha 1：该基因编码的蛋白质属于ser/thr蛋白激酶家族。它是 5'-prime-AMP 活化蛋白激酶 (AMPK) 的催化亚基。AMPK 是一种保存在所有真核细胞中的细胞能量感受器。AMPK 的激酶活性由增加细胞 AMP/ATP 比率的刺激物激活。AMPK 通过磷酸化调节许多关键代谢酶的活性。它通过关闭消耗 ATP 的生物合成途径来保护细胞免受导致 ATP 耗尽的压力。已经观察到编码不同亚型的可变剪接转录物变体。[由 RefSeq 提供，2008 年 7 月]

Free

Q13131

Predicted band size: 64 kDa; Observed band size: 64 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102192

Neuroscience

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Western blot analysis of extracts from HUVEC(lane 2(20μg) and HUVEC(lane 3(40μg) using Phospho-AMPK alpha 1 (Ser496) (HY-P80790) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C over-night. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-AMPK alpha 1 (Ser496) with Phospho-AMPK alpha 1 (Ser496) Antibody (HY-P80790)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-AMPK alpha 1 (Ser496) Antibody (HY-P80790) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-AMPK alpha 1 (Ser496) with Phospho-AMPK alpha 1 (Ser496) Antibody (HY-P80790) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-AMPK alpha 1 (Ser496) Antibody (HY-P80790) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser496 of Human AMPKα1.The exact sequence is proprietary to MCE.