RhoA/B/C 抗体

RhoA/B/C Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RhoA/B/C.

RhoA 蛋白是一种小型 GTP 酶，对于协调不同的细胞反应、在活跃的 GTP 结合状态和不活跃的 GDP 结合状态之间循环至关重要。RhoA 的活性形式主要与细胞骨架的组织相关，可与各种效应蛋白结合，影响细胞过程，例如细胞骨架动力学、细胞迁移和细胞周期进程。它在调节将质膜受体连接到粘着斑和肌动蛋白应力纤维组装的信号转导途径中发挥着关键作用。此外，RhoA 对于细胞周期胞质分裂期间肌球蛋白收缩环形成所需的微管依赖性信号是不可或缺的，有助于卵裂沟的形成。RhoA 对于角质细胞间粘附的顶端连接形成至关重要，还参与 MEMO1-RHOA-DIAPH1 信号通路，影响 ERBB2 依赖性微管稳定性。它进一步调节钾通道活性，调节鸟嘌呤核苷酸交换因子，并在微生物感染过程中充当鼠疫耶尔森氏菌 yopT 半胱氨酸肽酶的靶标。

Free

P61586 / P62745 / P08134

Predicted band size: 22 kDa; Observed band size: 22 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102989

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from NIH/3T3(lane 2(20ug) ,HepG2(lane 3(20ug) and Hela(lane 4(20ug) using RhoA/B/C Antibody (HY-P80882) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling RhoA/B/C with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling RhoA/B/C with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Collagen VI alpha 123 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Collagen VI alpha 123 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Rho A + B + C.The exact sequence is proprietary to MCE.